炭疽病对不同核桃品种抗性生理指标的影响  

Effects of Colletotrichum gloeosporioides on the Physiological Indexes of Resistance of Fifferent Walnut Varieties

作　　者：任得元[1] 高向倩 王田雨 惠建超 杨桂燕[2] REN De-yuan;GAO Xiang-qian;WANG Tian-yu;HUI Jian-chao;YANG Gui-yan(Yangling Vocational&Technical College,Yangling 712100,Shaanxi,China;College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区：[1]杨凌职业技术学院,陕西杨陵712100 [2]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2020年第2期137-141,共5页Journal of Northwest Forestry University

基　　金：中央财政林业科技推广项目“千阳核桃综合配套丰产栽培技术示范与推广”SLTG[2016]14号;陕西省博士后基金项目(2016BSHEDZZ117);陕西省自然科学基础研究计划项目(2018JQ3066);大学生创新创业训练计划项目(S201910712143)。

摘　　要：核桃是我国重要木本油料及经济树种,其生长发育、坚果产量及品质易受病害影响,其中炭疽病为核桃主要病害之一。因此,深入了解常见核桃品种响应炭疽病的能力,可为选育抗病性较强品种提供理论依据。对‘香玲’‘西洛3’‘西林3’‘契可’核桃分别进行炭疽病胁迫,分析胁迫下的活性氧(ROS)代谢以及抗氧化酶活性。结果表明,炭疽病菌胁迫前各植株生理指标差异均不明显,胁迫后各植株均表现为ROS(H 2O 2、O·-2)积累增多,膜脂过氧化(MDA)加剧,抗氧化酶(SOD、POD、CAT)活性增强。其中‘契可’MDA含量明显高于‘香玲’‘西洛3’‘西林3’;‘西林3’‘契可’的H 2O 2与O·-2含量明显高于‘香玲’‘西洛3’。而‘香玲’的SOD、POD、CAT活性明显高于‘西洛3’‘西林3’‘契可’,且活性增强幅度为‘香玲’>‘西洛3’>‘西林3’>‘契可’。由此可知‘香玲’‘西洛3’‘西林3’‘契可’核桃响应炭疽病的生理表现上为‘香玲’>‘西洛3’>‘西林3’>‘契可’。Juglans regia(walnut)is an important woody oil and non-timber tree species in China,which is susceptible to diseases,resulting negative impacts on the tree development,nut yield and quality.Anthracnose(Colletotrichum gloeosporioides)is one of the main diseases occurring in walnut tree.Therefore,an in-depth understanding of the ability of common walnut varieties to respond to anthracnose can provide a theoretical basis for breeding varieties with strong disease resistance.In this study,four walnut varieties‘Xiangling’,‘Xiluo 3’,‘Xilin 3’,and‘Qike’were anthracnose-stressed,respectively,and the active oxygen(ROS)metabolism and antioxidant enzyme activities were analyzed.The results showed that all the physiological indexes of all the selected species were not significantly different before the anthracnose stress.However,after stress,all plants showed an increase in ROS(H 2O 2,O·-2)accumulation,an intensification in membrane lipid peroxidation(MDA),and enhancement in antioxidant enzyme(SOD,POD,CAT)activities.Among these four varieties,the MDA content of‘Qike’was significantly higher than‘Xiangling’,‘Xiluo 3’,‘Xilin 3’;the H 2O 2 and O·-2 content of‘Xilin 3’and‘Qike’was significantly higher than‘Xiangling’and‘Xiluo 3’.However,the activities of SOD,POD and CAT of‘Xiangling’were significantly higher than‘Xiluo 3’,‘Xilin 3’and‘Qike’,and the range was‘Xiangling’>‘Xiluo 3’>‘Xilin 3’>‘Qike’.These results suggested that the physiological performance of these four walnut varieties in response to anthracnose were‘Xiangling’>‘Xiluo 3’>‘Xilin 3’>‘Qike’.

关 键 词：核桃 炭疽病 ROS积累 生理表现 

分 类 号：S763.1[农业科学—森林保护学]