通过ITS序列分析鉴定云南重楼内生真菌  

Identification of Endophytic Fungi by ITS Sequence Analysis in Paris polyphylla var.yunnanensis

作　　者：张晓云 任可 李维蛟 ZHANG Xiao-yun;REN Ke;LI Wei-jiao(Yunnan University of Traditional Chinese Medicine/Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Tradition Chinese Medicine Materials,Kunming 650500,China;Yunnan Agricultural University,Kunming 650500,China)

机构地区：[1]云南中医药大学/云南省道地濒危中药材繁育与栽培工程技术研究中心,云南昆明650500 [2]云南农业大学,云南昆明650500

出　　处：《江西农业学报》2020年第3期42-47,共6页Acta Agriculturae Jiangxi

基　　金：“代谢组学与病症解的自身免疫性肝炎诊断模式构建”(81760812);云南省应用基础研究项目“基于代谢组学的AIH-PBC重叠综合征中医证型研究”[2018FF001(-021)];云南中医药大学中药学院学科建设项目(2019ZY014);云南省科学技术厅“云南省道地濒危中药材繁育与栽培工程技术研究中心”(2016DH011);云南省重大科技专项(生物医药)(2018ZF011-0202)。

摘　　要：采用组织分离法从云南重楼(Paris polyphylla var.yunnanensis)根、根状茎和叶3个部位的组织块中分离出内生真菌,并对这些内生真菌进行了传统形态学和分子生物学(ITS序列)鉴定。共分离到内生真菌26株,鉴定为8属9种,其中从叶中分离出24株,从根中分离出2株,鉴定为1属1种,即球毛壳菌(Chaeto miumglobosum)。在分离出的9种内生真菌中,优势菌种为1号菌和3号菌,分别被鉴定为尖孢镰刀菌(Fusarium oxysporum)和博宁炭疽菌(Colleto trichumboninense),分离率分别为23.07%和19.23%。Endophytic fungi were isolated from roots,rhizomes and leaves of Paris polyphylla Smith var.yunnanensis by tissue isolation method,and identified by ITS sequence.A total of 26 strains of endophytic fungi were isolated and identified as 8 genera and 9 species.Twenty-four strains were isolated from leaves,2 strains were isolated from roots,identified as 1 genus and 1 species,and identified as C.globosum by molecular identification.The dominant strains were No.1 and No.3.The isolation rates of F.oxysporum and C.boninense were 23.07%and 19.23%,respectively.

关 键 词：云南重楼 内生真菌 ITS序列 鉴定 

分 类 号：S432.44[农业科学—植物病理学]