白藜芦醇对气态甲醛诱导大鼠脑损伤及神经颗粒素表达的影响  

Resveratrol prevents brain injury and neurogranin expression in rats exposed to gaseous formaldehyde

作　　者：彭莉 张祥[1] 李仲铭[2] 范炜[2] 刘杏 魏晓菡 邓洁[2] Peng Li;Zhang Xiang;Li Zhongming;Fan Wei;Liu Xing;Wei Xiaohan;Deng Jie(Experimental Center of Basic Medicine,Kunming Medical University,Kunming 650500,China;Department of Anatomy and Histo-embryology,Kunming Medical University,Kunming 650500,China)

机构地区：[1]昆明医科大学基础医学实验教学中心,650500 [2]昆明医科大学人体解剖学与组织胚胎学系,650500

出　　处：《中华行为医学与脑科学杂志》2020年第2期97-101,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目(81460492)。

摘　　要：目的探讨气态甲醛蓄积对大鼠学习记忆能力及前额皮层、海马区神经颗粒素(neurogranin,Ng)表达的影响,研究白藜芦醇(resveratrol,Res)对气态甲醛蓄积所致脑损伤的防治效果。方法成年雄性SD大鼠84只,每组12只完全随机分组法分为对照组(N组)、气态甲醛蓄积低浓度组(FL组)、气态甲醛蓄积中浓度组(FM组)、气态甲醛蓄积高浓度组(FH组)、气态甲醛蓄积低浓度+白藜芦醇组(FLR组)、气态甲醛蓄积中浓度+白藜芦醇组(FMR组)、气态甲醛蓄积高浓度+白藜芦醇组(FHR组),每组12只。采用气态甲醛暴露法建立甲醛染毒动物模型,运用Morris水迷宫检测大鼠学习记忆功能,采用蛋白免疫印迹法检测前额皮层、海马Ng表达与分布。结果(1)与N组大鼠逃避潜伏期、原平台滞留时间[(19.75±4.08)s,(43.47±2.43)s]相比,FM、FH组大鼠逃避潜伏期均延长[(25.52±2.98)s、(27.45±2.39)s,t=-4.97,-5.82;均P<0.01],原平台滞留时间均缩短[(30.99±4.21)s、(18.70±2.58)s,t=10.24,25.71;均P<0.01]。与FM、FH组等对应甲醛蓄积组相比,FMR、FHR组大鼠逃避潜伏期均明显延长[(21.37±3.80)s、(24.26±3.56)s,t=1.86,3.93;均P<0.05],原平台滞留时间明显延长[(40.33±3.63)s、(23.46±4.73)s,t=-7.50,-3.95;均P<0.01]。(2)与N组大鼠皮层、海马Ng表达[(0.99±0.03),(1.07±0.03)]相比,FM、FH组大鼠皮层、海马Ng蛋白表达显著降低[(0.62±0.06),(0.39±0.03),(0.77±0.09),(0.46±0.10),t=11.55,14.08,11.51,13.17;均P<0.01]。与FM、FH组等对应甲醛蓄积组相比,FMR、FHR组大鼠皮层、海马Ng表达均有明显升高[(0.94±0.11),(0.48±0.04),(0.95±0.05),(0.60±0.09),t=-5.26,-2.09,-5.32,-2.21;均P<0.05]。结论白藜芦醇能改善气态甲醛蓄积所致大鼠学习记忆功能减退,其机制可能是与Ng表达上调有关。Objective To explore the effects of gaseous formaldehyde exposure on male Sprague-Dawley rat brain function and synaptic protein expression,as well as the neuroprotective effects of resveratrol.Methods Eighty-four male Sprague-Dawley rats were randomly divided into seven group with 12 in each group:control group(N),gaseous formaldehyde accumulation low concentration group(FL group),gaseous formaldehyde accumulation medium concentration group(FM group),gaseous formaldehyde accumulation high concentration group(FH group),gaseous formaldehyde accumulation low concentration+resveratrol group(FLR group),gaseous formaldehyde accumulation concentration+resveratrol group(FMR group)and gaseous formaldehyde accumulation high concentration+resveratrol group(FHR group).The animal model of gaseous formaldehyde accumulation was established by gas formaldehyde exposure method.Learning and memory function was tested by Morris water maze.Cortical and hippocampal neurogranin(Ng)protein expression in brain cortex and hippocampus was measured by Western blot.Results(1)Compared with the escape latency and original platform retention time of rats in group N((19.75±4.08)s,(43.47±2.43)s),the escape latency of rats in FM and FH groups was prolonged((25.52±2.98)s,(27.45±2.39)s,t=-4.97,-5.82;both P<0.01),the retention time of the original platform was shortened((30.99±4.21)s,(18.70±2.58)s,t=10.24,25.71;both P<0.01).Compared with the corresponding formaldehyde accumulation groups such as the FM and FH groups,the escape latency of the rats in the FMR and FHR groups was significantly longer((21.37±3.80)s,(24.26±3.56)s,t=1.86,3.93;both P<0.05),the retention time of the original platform was significantly prolonged((40.33±3.63)s,(23.46±4.73)s,t=-7.50,-3.95;both P<0.01).(2)Compared with the expression of Ng in cortex and hippocampus of rats in group N((0.99±0.03),(1.07±0.03)),the expression of Ng protein in cortex and hippocampus of rats in FM and FH groups was significantly reduced((0.62±0.06),(0.39±0.03),(0.77±0.09),(0.46±0.10)

关 键 词：气态甲醛蓄积 神经颗粒素 学习记忆 白藜芦醇 大鼠 

分 类 号：R28[医药卫生—中药学]