四环素耐药无乳链球菌生物膜形成特点  

Biofilm formation in tetracycline-resistant clinical Streptococcus agalactiae isolates

作　　者：赵琳珊 徐广健 陈俊文 孙翔 邓启文[1] 余治健[1] 李多云[1] ZHAO Linshan;XU Guangjian;CHEN Junwen;SUN Xiang;DENG Qiwen;YU Zhijian;LI Duoyun(Infectious Diseases,Nanshan People′s Hospital,Shenzhen Key Laboratory for Endogenous Infections,Shenzhen,Guangdong 518052,China)

机构地区：[1]深圳市南山区人民医院感染科,深圳市内源性感染研究重点实验室,广东深圳518052

出　　处：《中国热带医学》2020年第3期227-230,共4页China Tropical Medicine

基　　金：深圳市科创委学科布局项目(No.JCYJ20180508162403996);深圳市基础研究项目(No.JCYJ20180302144721183)。

摘　　要：目的本研究旨在探讨无乳链球菌生物膜形成能力及其与四环素耐药情况、四环素耐药基因、毒力基因之间的关系。方法收集深圳市南山区人民医院2014—2017年无乳链球菌临床分离株共136株。用结晶紫染色法检测无乳链球菌的生物膜形成情况。采用聚合酶链反应(PCR)的方法检测四环素耐药基因(tetM、tetK、tetO)及毒力基因(bca、bac、fbsA、fbsB、cpsA、scpB、rib、cpsⅢ、bfb、hylB、lmb、cylE)。结果136株无乳链球菌临床分离株生物膜形成率为39.7%(54/136),四环素敏感、中介和耐药的无乳链球菌生物膜形成率分别为37.0%(27/73)、0.0%(0/0)和42.9%(27/63);生物膜形成与无乳链球菌四环素耐药性无显著相关性(P>0.05);四环素耐药基因(tetM、tetK、tetO)与生物膜形成无显著相关性;全部菌株均未检测到bac基因,全部菌株检测到fbsB、bfb、hylB、lmb、cylE基因,毒力基因bca、fbsA、cpsA、scpB、rib、cpsⅢ与生物膜形成能力显著相关。结论无乳链球菌生物膜形成与四环素耐药无显著相关,与毒力基因bca、fbsA、cpsA、scpB、rib、cpsⅢ等可能相关。Objective This study investigated characteristics of biofilm formation in tetracycline-resistant clinical Streptococcus agalactiae(S.agalactiae)isolates,as well as its relationship with resistance genes,virulence factors and MLST.Methods A sample of 136 S.agalactiae isolates were collected retrospectively in Nanshan People's Hospital,Shenzhen,2014-2017.Crystal violet stainingwas performed to detect biofilms.Resistance genes(tetM,tetK,tetO)and virulence factors(bca,bac,fbsA,fbsB,cpsA,scpB,rib,cpsⅢ,bfb,hylB,lmb,cylE)were detected by polymerase chain reaction(PCR)analysis.Results The prevalence of biofilm formation among the clinical S.agalactiae isolates was 39.7%(54/136).The biofilm formation rates of tetracycline sensitive,intermediate and resistant in S.agalactiae were 37.0%(27/73),0.0%(0/0)and 42.9%(27/63),respectively.There was no significant correlation between biofilm formation and tetracycline resistance in S.agalactiae(P>0.05).Tetracycline resistance genes(tetM,tetK,tetO)had no significant correlation with biofilm formation.Bac gene was not detected in all strains,while fbsB,bfB,hylB,lmb and cylE genes were detected in all strains.Virulence genes bca,fbsA,cpsA,scpB,rib and cpsⅢwere significantly correlated with biofilm formation ability.Conclusion The antimicrobial activity of tetracycline against clinical Streptococcus agalactiae isolates is not associated with biofilm formation.Virulence factors(bca,fbsA,scpB,rib and cpsⅢ)were associated with the biofilm formation in S.agalactiae isolates.

关 键 词：无乳链球菌 生物膜 四环素 

分 类 号：R378.12[医药卫生—病原生物学]