基于ATR-FTIR结合UPLC-DAD技术鉴别百合及其类似品  

Identification of Lilii Bulbus and Its Counterfeit by ATR-FTIR and UPLC-DAD

作　　者：袁志鹰[1,2,3] 李东洋 李亚林 管贺 刘湘丹 谢梦洲[1,3] 黄惠勇[1,3] YUAN Zhiying;LI Dongyang;LI Yalin;GUAN He;LIU Xiangdan;XIE Mengzhou;HUANG Huiyong(Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Engineering Technology Center of Standardization and Function of Chinese Herbal Decoction Pieces,Changsha 410208,China;Hunan Engineering Technology Center of Functional Food Homology of Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省中药饮片标准化及功能工程技术研究中心,湖南长沙410208 [3]湖南省药食同源功能性食品工程技术研究中心,湖南长沙410208

出　　处：《中国中医药信息杂志》2020年第1期57-62,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家中药标准化项目(ZYBZH-Y-HUN-24);国家中医药管理局项目(201728);湖南省自然科学基金(2019JJ50446);长沙市科技计划(kq1801044);湖南省中药饮片标准化及功能工程技术研究中心开放基金(2018-02-01);湖南中医药大学中药学一流学科(2018年)。

摘　　要：目的基于傅里叶变换衰减全反射红外光谱(ATR-FTIR)结合超高效液相色谱-二极管阵列检测器(UPLC-DAD)技术快速区分不同品种百合药材粉末。方法采用ATR-FTIR采集百合样品红外光谱数据并导入Simca软件,建立正交偏最小二乘判别分析(OPLS-DA)模型,在此基础上,联用UPLC-DAD技术测定正品百合、伪品金百合及微波干燥卷丹中王百合苷A含量,分析其差异性。结果OPLS-DA二维得分图及层次聚类分析图对卷丹与百合、卷丹与伪品金百合、热风干燥卷丹与微波干燥卷丹进行了良好的分类。卷丹的王百合苷A含量为2.09~3.28 mg/g,百合的王百合苷A含量为1.36~1.77 mg/g,伪品金百合的王百合苷A含量为1.03~1.05 mg/g,微波干燥卷丹的王百合苷A含量为3.92~4.01 mg/g。卷丹和百合中的王百合苷A含量高于伪品金百合,微波干燥卷丹的王百合苷A含量高于普通热风干燥卷丹。结论本研究建立的方法操作简单、方便、快速,可为百合饮片、百合药膳产品开发及相关药材的质量控制提供借鉴。Objective To quickly distinguish different kinds of Lilii Bulbus powder based on ATR-FTIR and UPLC-DAD.Methods The infrared spectral data of samples were collected by ATR-FTIR,and the data was imported into Simca software to establish the orthogonal partial least squares discriminant analysis(OPLS-DA)model.On this basis,UPLC-DAD was used to determine the content of regaloside A in genuine Lilii Bulbus and Lilium trompeten(counterfeit)and microwave dried Lilium lancifolium Thunb.,and the differences among different samples were analyzed.Results Two-dimensional score map of OPLS-DA and hierarchical cluster analysis were used to classify the three groups of Lilium lancifolium Thunb.and Lilium broumii F.E.Brown var.viridulum Baker,Lilium lancifolium Thunb.and Lilium trompeten(counterfeit),hot-air dried Lilium lancifolium Thunb.and microwave dried Lilium lancifolium Thunb.The content of regaloside A in Lilium lancifolium Thunb.was 2.09–3.28 mg/g,1.36–1.77 mg/g in Lilium broumii F.E.Brown var.viridulum Baker,1.03–1.05 mg/g in Lilium trompeten(counterfeit),and 3.92–4.01 mg/g in microwave Lilium lancifolium Thunb.The content of regaloside A in Lilium lancifolium Thunb.and Lilium broumii F.E.Brown var.viridulum Baker was significantly higher than that in the counterfeit,while the content of regaloside A in microwave dried Lilium lancifolium Thunb.was higher than that in the hot-air dried Lilium lancifolium Thunb.Conclusion The method is simple,convenient and rapid,which can provide references for the development of medicinal diet products of Lilii Bulbus and the quality control of related medicinal materials.

关 键 词：百合 傅里叶变换衰减全反射红外光谱 超高效液相色谱-二极管阵列检测器 王百合苷A 

分 类 号：R284.1[医药卫生—中药学]