肺结核病患者外周血α-防御素HNP1-3的表达及意义  

Expression and significance ofα-defensin HNP1-3 in human peripheral blood of patients with tuberculosis

作　　者：罗槑 刘勇 罗东霞 徐园红 朱玛 黄涛 LUO Mei;LIU Yong;LUO Dong-xia;XU Yuan-hong;ZHU Ma;HUANG Tao(Infectious Diseases Laboratory,Public Health and Clinical Center of Chengdu,Chengdu,Sichuan 610066,China)

机构地区：[1]成都市公共卫生临床医疗中心传染病研究室,四川成都610066 [2]成都市公共卫生临床医疗中心检验科,四川成都610066 [3]成都市公共卫生临床医疗中心结核科,四川成都610066

出　　处：《临床肺科杂志》2020年第4期496-500,共5页Journal of Clinical Pulmonary Medicine

基　　金：四川省2017年科技计划项目(No.2017JY0090);成都市2015年科技惠民项目(No.2015-HM01-00074-SF);成都市卫计委2015年医学科研课题(No.2015054)。

摘　　要：目的探讨肺结核病患者外周血α-防御素HNP1-3水平与疾病的关系。方法共纳入研究对象199例,其中肺结核患者113例,健康对照86例。运用酶联免疫吸附实验(ELISA)、实时荧光定量(qPCR)等方法检测研究对象外周血HNP1-3水平及外周血中性粒细胞HNP1-3的mRNA表达水平。进一步将结核组分为病原学阳性组TB(+)组和病原学阴性组TB(-)、初治肺结核组(NTB)和复治肺结核组(RTB)、重症肺结核组(Severe)与轻症肺结核组(Mild)、肺结核组(TB)与肺结核合并糖尿病组(TB+DM),并比较各组HNP1-3水平情况。结果肺结核组外周血HNP1-3为(22.6±14.4)ng/mL,显著高于健康对照组(16.1±5.7)ng/mL(t=4.008、P<0.001);肺结核组中性粒细胞HNP1-3的mRNA表达为健康对照组2.17倍,差异无统计学意义(t=2.221、P=0.051);TB(+)组与TB(-)组外周血HNP1-3水平分别为(23.5±14.5)ng/mL、(20.0±10.4)ng/mL,差异无统计学意义(t=1.360、P=0.175);NTB组与RTB组外周血HNP1-3水平分别为(22.1±13.7)ng/mL、(23.8±15.7)ng/mL,差异无统计学意义(t=0.596、P=0.552);重症组外周血HNP1-3水平(25.2±16.3ng/mL)显著高于轻症组(19.5±11.0ng/mL),两组差异有统计学意义(t=2.098、P=0.038);肺结核合并糖尿病组外周血HNP1-3水平(28.6±13.9)ng/mL显著高于未合并糖尿病肺结核组(20.9±14.1)ng/mL,两组差异有统计学意义(t=2.398、P=0.018)。肺结核患者外周血HNP1-3水平与IL-8及中性粒细胞比例呈正相关。结论结核病患者HNP1-3水平高于健康人群,且与疾病病情、预后及炎症程度密切相关。Objective To explore the relationship betweenα-defensin HNP1-3 in human peripheral blood of patients with tuberculosis and disease progress.Methods A total of 113 pulmonary tuberculosis patients and 86 healthy controls were enrolled.The HNP1-3 level was detected by ELISA,and the expression of HNP1-3 mRNA in peripheral neutrophils was analyzed by qPCR.Patients with tuberculosis were further divided into the TB(+)group and the TB(-)group,the NTB group and the RTB group,the severe group and the mild group,the TB group and the TB+DM group,and the levels of HNP1-3 were compared.Results The level of HNP1-3 was increased significantly in the tuberculosis group than the healthy control group[(22.6±14.4)ng/mL vs(16.1±5.7)ng/mL,t=4.008,P<0.001].The expression of HNP1-3 mRNA in peripheral neutrophils was increased 2-fold in the tuberculosis group than the healthy control group,but there was no significant difference between the two groups(t=2.221,P=0.051).There was no significant difference in the level of HNP1-3 between the TB(+)group and the TB(+)group(23.5±14.5 ng/mL vs 20.0±10.4 ng/mL,t=1.360,P=0.175).There was no significant difference in the level of HNP1-3 between the NTB group and the RTB group(22.1±13.7 ng/mL vs 23.8±15.7 ng/mL,t=0.596,P=0.552).The levels of HNP1-3 level was increased significantly in the severe group than the mild group(25.2±16.3 ng/mL vs 19.5±11.0 ng/mL,t=2.098,P=0.038).The levels of HNP1-3 level was increased significantly in the TB+DM group than the TB group(28.6±13.9 ng/mL vs 20.9±14.1 ng/mL,t=2.398,P=0.018).The levels of HNP1-3 in peripheral blood was positively correlated with IL-8 and neutrophil ratio in patients with tuberculosis.Conclusion The level of HNP1-3 in tuberculosis patients increases more significantly than the healthy control group,and it is closely related to illness situations,prognosis,and inflammation.

关 键 词：Α-防御素 人中性粒细胞肽1-3 肺结核 炎症 

分 类 号：R521[医药卫生—内科学]