IL-8及SPB蛋白在兔海水及淡水淹溺型急性肺损伤模型中的表达  

Expression of IL-8 and SPB protein in rabbit with acute lung injury induced by seawater and freshwater drowning

作　　者：乔永平 刘轶宁 QIAO Yong-ping;LIU Yi-ning(Department of Respiratory Medicine,the 967th Hospital of PLA Joint Services,Dalian,Liaoning 116001,China)

机构地区：[1]中国人民解放军联勤保障部队第九六七医院,中山大连116001

出　　处：《临床肺科杂志》2020年第4期574-577,609,共5页Journal of Clinical Pulmonary Medicine

摘　　要：目的探究组织白细胞介素(IL-8)及表面活性蛋白B(SPB)在兔海水及淡水淹溺型急性肺损伤模型中的表达。方法选取实验中心饲养健康成年新西兰兔36只,采用随机数表分组,将其分海水组,淡水组与对照组。三组兔均在动物房饲养一周后行麻醉下气管插管,气管插管完成后,海水组兔经气管插管向气管内注射3 mL/kg配置海水,淡水组经气管插管向气管内注射18 mL/kg配置淡水,对照组兔仅行麻醉气管插管操作。手术操作完成后,持续插管通气4 h,通气结束后处死各组实验兔,收集肺组织,光学显微镜下观察各组兔肺组织病理学改变情况,测定肺组织干湿重比(W/D);比较三组兔肺泡灌注液中IL-8及SPB蛋白表达情况;比较三组兔肺组织IL-8及SPB蛋白表达情况。结果海水组与淡水组W/D较对照组均明显上升,结果具有统计学意义(P<0.05),且两组兔肺组织W/D无明显差异(P>0.05);海水组与淡水组肺泡灌洗液中IL-8水平较对照组表达均上升,SPB蛋白较对照组明显下降,结果具有统计学意义(P<0.05),且两组兔肺泡灌洗液中IL-8及SPB蛋白水平无明显差异(P>0.05);海水组与淡水组肺组织中IL-8水平较对照组表达均上升,SPB水平较对照组明显下降(P<0.05),且两组兔肺组织中IL-8及SPB蛋白表达无明显差异(P>0.05)。结论海水组及淡水组淹溺型急性肺损伤实验兔肺泡灌洗液及肺组织IL-8水平均明显上升,SPB水平均明显下降,提示IL-8表达增多及SPB蛋白表达下调与该损伤发生过程密切相关。Objective To investigate the expression of interleukin-8(IL-8)and surfactant protein B(SPB)in rabbit with acute lung injury induced by seawater and freshwater drowning.Methods 36 healthy adult New Zealand rabbits were randomly divided into the seawater group,the freshwater group and the control group.All rabbits were fed in the animal room for one week and then intubated under anesthesia.After the intubation was completed,the seawater group was injected with 3 mL/kg seawater into the trachea through the tracheal intubation.The freshwater group was injected with 18 mL/kg fresh water into the trachea through the tracheal intubation.The control group was only given anesthetic tracheal intubation.After the operation,the rabbits in each group were sacrificed after 4 hours of continuous intubation and ventilation.Lung tissues were collected.The pathological changes of lung tissues in each group were observed under light microscope,and the dry-wet weight ratio(W/D)of lung tissues was measured.The expressions of IL-8 and SPB in alveolar lavage fluid and lung tissues of the three groups were compared.Results The value of W/D in lung tissues of the seawater group and the freshwater group was significantly higher than that in the control group(P<0.05),and there was no significant difference in W/D between the two groups(P>0.05).The expression of IL-8 in alveolar lavage fluid of the seawater group and the freshwater group was higher than that of the control group,and SPB protein was significantly lower than that of the control group The expression levels of IL-8 and SPB in alveolar lavage fluid of the two groups were not significantly different(P>0.05).The expression levels of IL-8 and SPB in pulmonary tissues of the seawater group and the freshwater group were higher than those of the control group,and the level of SPB was significantly lower than that of the control group(P<0.05),and the results were statistically significant(P<0.05).There was no significant difference in the expression of IL-8 and SPB in rabbit lung tissu

关 键 词：IL-8 SPB 淹溺型急性肺损伤 肺组织干湿重比 

分 类 号：R563[医药卫生—呼吸系统] R-332[医药卫生—内科学]