温敏核不育水稻HD9802-9S育性相关基因的BSA测序初步分析  

Prelimary analysis of BSA-seq of fertility related genes in thermo-sensitive genic male sterile rice HD9802-9S

作　　者：甘丹阳 李龙盘 王倩 徐国成[1] 居超明[1] GAN Danyang;LI Longpan;WANG Qian;XU Guocheng;JU Chaoming(College of Life Sciences,Hubei University,Wuhan 430062,China)

机构地区：[1]湖北大学生命科学学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2020年第2期165-171,共7页Journal of Hubei University：Natural Science

基　　金：湖北省技术创新重大项目(2018ABA083)资助。

摘　　要：HD9802-9S是以HD9802S为母本、以固优12为父本杂交选育的一个温敏核不育系.人工气候箱鉴定该不育系转育起始温度高于HD9802S,表明HD9802-9S和HD9802S两个品系的育性调控基因有差异.构建HD9802-9S/R446 F 2群体和HD9802-9S/R144 F 2群体,每个群体中随机选择100个不育单株和100个高度可育单株分别构建可育混池和不育混池进行BSA重测序.统计并计算每个BSA重测序结果的ΔSNP-index值,并以95%和99%置信水平作为筛选的阈值.高于95%置信水平的ΔSNP-index定位于2号染色体上总长为3.24 Mb的候选区域和7号染色体上0.69 Mb长度的候选区域内;高于99%置信水平的ΔSNP-index定位于2号染色体上总长为1.87 Mb的候选区域内,在7号染色体上没有候选SNP,两个群体的定位结果相同.因此,HD9802-9S的不育主效基因定位在2号染色体,7号染色体上可能存在其他控制育性的QTL位点.HD9802-9S的2号染色体上存在64个控制花粉育性表达的候选基因.这些结果为进一步研究它们与tms 5之间的关系奠定基础.HD9802-9S was a thermo-sensitive male sterile line generated by crossing HD9802S as female parent and Guyou 12 as male parent.The critical sterility-inducing temperature of HD9802-9S was higher than that of HD9802S in the phytotron,which indicated the minor gene(s)involved in regulating thermos-sensitive male sterility were different between HD9802-9S and HD9802S.To identify the fertility related genes of HD9802-9S,HD9802-9S/R446 F 2 population and HD9802-9S/R144 F 2 population were constructed.In each population,two separate bulked DNA pools were constructed with DNAs extracted from either 100 randomly selected sterile plants or from 100 highly fertile plants.The bulked segregant analysis(BSA)was used to identify candidates for regions associated with male sterility of HD9802-9S.The parameter,ΔSNP-index which reflects the proportion of reads harboring the SNP that different from the reference genome was calculated to identify candidate region for fertility related genes in HD9802-9S.We found the F 2 progeny revealed a cluster of SNP in a region(3.24 Mb)on chromosome 2 and a region(0.69 Mb)on chromosome 7 with 95%confidence intervals ofΔSNP-index,or a cluster of SNP in a region(1.87 Mb)on chromosome 2 and no candidate on chromosome 7 with 99%confidence intervals ofΔSNP-index.Moreover,the candidate regions associated with male sterility of the two populations showed a high degree of consistency.The main sterile gene of HD9802-9S is located on chromosome 2,and there may be other QTL loci controlling fertility on chromosome 7.There are four candidate genes controlling pollen fertility in HD9802-9S.These results lay a foundation for further study of the relationship between them and tms 5.

关 键 词：水稻 温敏核不育基因 BSA 基因定位 

分 类 号：Q37[生物学—遗传学]