两种处理方式对小鼠卵巢组织玻璃化冷冻效果的影响  

EFFECTS OF TWO TREATMENTS ON VITRIFICATION OF MOUSE OVARIAN TISSUE

作　　者：刘小超 钮淋 赵雪莹 王凤霞 司丽娜[1] 杨松鹤[1] LIU Xiao-chao;NIU lin;ZHAO Xue-ying(Department of Human Anatomy,Chengde Medical College,Hebei Chengde 067000,China)

机构地区：[1]承德医学院人体解剖学教研室,河北承德067000

出　　处：《承德医学院学报》2020年第2期95-98,共4页Journal of Chengde Medical University

摘　　要：目的:研究片段冻存及整体冻存小鼠卵巢组织对玻璃化冷冻效果的影响。方法:取小鼠卵巢组织,随机分为片段冻存组、整体冻存组和新鲜对照组。新鲜对照组卵巢取出后立即固定于4%多聚甲醛中,片段冻存组卵巢切割成1×1×1mm^3大小后冻存,整体冻存组卵巢不进行切割处理直接冻存。各组小鼠卵巢常规石蜡包埋,切片后HE染色。计数各组卵巢形态正常卵泡数,计算形态正常卵泡率,测量各组各类卵泡直径。结果:整体冻存组、片段冻存组正常卵泡率明显低于新鲜对照组(P<0.01);整体冻存组、片段冻存组正常卵泡率比较,差异无统计学意义(P>0.05)。整体冻存组、片段冻存组原始卵泡、初级卵泡、次级卵泡、窦状卵泡直径均明显高于新鲜对照组(P<0.05);整体冻存组、片段冻存组各类卵泡直径比较,差异均无统计学意义(P>0.05)。结论:整体冻存小鼠卵巢组织不影响玻璃化冷冻的效果。Objective:To compare the effects of fragment cryopreservation of mouse ovarian tissue and whole cryopreservation of mouse ovarian tissue on vitrification.Methods:Mouse ovarian tissues were randomly divided into fragment cryopreservation group,whole cryopreservation group and fresh control group.The ovarian tissue of mice in fresh control group were immediately fixed with 4%paraformaldehyde after taking out;The ovarian tissue of mice in fragment cryopreservation group were cryopreserved after cut into 1×1×1 mm^3 cubes;The ovarian tissue of mice in whole cryopreservation group were cryopreserved directly with no cutting.The rate of normal morphological follicles were calculated and the diameter of all kinds of follicles were measured after the ovarian tissues embedding and HE staining as normal.Results:The normal morphological follicles rate of ovarian tissues in fragment cryopreservation group and whole cryopreservation group were significantly lower than fresh control group(P<0.01);There had no statistical significance about normal morphological follicles rate between fragment cryopreservation group and whole cryopreservation group(P>0.05).The diameter of Primordial follicle,primary follicle,secondary follicle,sinus follicle of ovarian tissues in fragment cryopreservation group and whole cryopreservation group were significantly higher than fresh control group(P<0.05);There had no statistical significance about diameter of all kinds of follicles between fragment cryopreservation group and whole cryopreservation group(P>0.05).Conclusions:Whole cryopreservation of mouse ovarian tissue does not affect the effects of vitrification.

关 键 词：小鼠 卵巢 玻璃化冷冻 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]