槐米中芦丁的提取工艺优化及检识  

Optimization of extraction technology and detection of rutin from sophora japonica

作　　者：张依 周泽纯 全锦秋 蒙兰花 ZHANG Yi;ZHOU Zechun;QUAN Jinqiu;MENG Lanhua(College of Pharmacy,Guangzhou Medical University,Guangdong,Guangzhou 511436,China;School of Public Health,Guangzhou Medical University,Guangdong,Guangzhou 511436,China)

机构地区：[1]广州医科大学药学院,广东广州511436 [2]广州医科大学公共卫生学院,广东广州511436

出　　处：《中国医药科学》2020年第3期38-41,共4页China Medicine And Pharmacy

基　　金：广东省广州市教育科学规划课题(201811666);广州医科大学大学生科技创新项目(2018A001)。

摘　　要：目的优化槐米的提取工艺以提高芦丁的提取率。方法在碱提酸沉的基础上,选用电热套加热(130℃,微沸)回流30min,再超声辅助(70℃,40kHz,200W)提取30min的方法提取芦丁。对影响芦丁提取率的因素如pH值、料液比和静置时间进行了考察。应用UV法和HPLC法对自提芦丁进行了检识。结果最优提取工艺为碱液溶解时pH值为8,料液比为1∶20(g/mL),酸沉淀后静置时间为24h。UV法和HPLC法检识结果表明此法自提的芦丁较纯。结论优化提取工艺可提高槐米中芦丁的提取率。Objective To optimize the extraction technology of sophora japonica to improve the extraction rate of rutin.Methods On the basis of alkali extraction and acid precipitation,rutin was extracted by electric heating(130℃,slightly boiling)for reflux for 30 min,and ultrasonic assisted extraction(70℃,40kHz,200W)for 30 min.Factors influencing the extraction rate of rutin such as pH value,solid-liquid ratio and standing time were investigated.UV method and HPLC method were used to detect rutin.Results The pH value of dissolved lye in the optimal extraction process was 8,the ratio of material to liquid was 1∶20(g/mL),and the acid precipitation time was 24 hours.The results of UV and HPLC showed that the rutin extracted by this method was relatively pure.Conclusion Optimized extraction technology can improve the extraction rate of rutin from sophora japonica.

关 键 词：槐米 芦丁 提取工艺 提取率 

分 类 号：R284.2[医药卫生—中药学]