兰州百合多糖BHP-1的化学结构与形貌分析  

Chemical Structure and Morphological Features of A Polysaccharide BHP-1 Purified from Bulbs of Lilium davidii var.unicolor

作　　者：惠和平 金辉[1] 杨晓燕[1] 李秀壮[1] 辛爱一[1] 秦波 HUI He-ping;JIN Hui;YANG Xiao-yan;LI Xiu-zhuang;XIN Ai-yi;QIN Bo(Key Laboratory of Chemistry of Northwestern Plant Resources,CAS/Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China;University of Chinese Academy of Sciences,Beijing 100049,China;Gansu Agriculture Technology College,Lanzhou 730020,China)

机构地区：[1]中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室,兰州化学物理研究所,兰州730000 [2]中国科学院大学,北京100049 [3]甘肃农业职业技术学院,兰州730020

出　　处：《中国实验方剂学杂志》2020年第8期170-175,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点研发计划项目(2017YFD0200804-05);国家自然科学基金项目(31570354,21775154,31772668,31560037)。

摘　　要：目的:以兰州百合鳞茎中分离和纯化得到的多糖BHP-1为主要研究对象,综合运用仪器分析和化学反应等手段研究其初步的形貌特征和化学结构。方法:采用热重分析(TG)及扫描电镜(SEC)技术分别分析多糖BHP-1的热稳定性和形貌结构特征;通过气相色谱-质谱联用(GC-MS)技术,结合部分酸水解、高碘酸钠氧化-Smith降解以及核磁共振波谱(NMR)分析等方法对多糖BHP-1的化学结构进一步的进行解析。结果:BHP-1是以1,4连接的α-D-吡喃葡萄糖和1,4连接的β-D-吡喃甘露糖为基本骨架的甘露葡聚糖,在葡萄糖和甘露糖的2位和/或3位形成主要的分支,主链或支链的末端残基主要为T-α-D-吡喃葡萄糖,同时在其结构片段中含有少量的O-乙酰基。热重分析显示BHP-1在220℃开始发生降解,520℃基本结束,说明BHP-1热稳定性良好。形貌分析显示BHP-1表面光滑有大量凹陷,凹陷处多为片层结构错落紧密堆积而成,并交织下陷呈不规则的孔洞。结论:多糖BHP-1是一种热稳定性良好,表面光滑有大量的凹陷和孔洞,并含少量O-乙酰基的甘露葡聚糖,其化学结构为首次报道。Objective:To study the chemical structure and morphological features of a polysaccharide BHP-1 isolated and purified from the bulbs of Lilium davidii var.unicolor.Method:Thermogravimetry(TG)and scanning electron microscopy(SEC)was used to determine the thermal characteristics and the surface morphology changes of the polysaccharide BHP-1,respectively.The chemical structure of BHP-1 was analyzed by gas chromatography-mass spectrometer(GC-MS),partial acid hydrolysis,sodium periodate oxidation-Smith degradation and nuclear magnetic resonance(NMR).Result:The results showed that the backbone of the polysaccharide BHP-1 mainly containedα-(1→4)-linked D-glucopyranosyl andβ-(1→4)-linked Dmannopyanosyl,and the branches were probably linked at the O-2 and/or O-3 of the mannosyl and glucosyl residues,with T-α-D-glucopyranosyl as a terminal structure.It was a natural mannoglucose containing a small amount of O-acetyl group.BHP-1 began to degrade at 220℃,and the degradation ended at 520℃,indicating a good thermal stability,smooth surface and a large number of depressions.The depressions were formed by closely interlaced and sunken sheet layers with irregular holes.Conclusion:BHP-1 was a natural mannoglucose with a good thermal stability,smooth surface,a large number of depressions and irregular holes,and contained a small amount of O-acetyl group.Its chemical structure was reported for the first time.

关 键 词：兰州百合 多糖 化学结构 热重与形貌分析 甘露葡聚糖 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学]