响应月季花朵衰老的RhRRs基因筛选及表达特性分析  

Screening and Expression Analysis of RhRRs Responding to Rose Flower Senescence

作　　者：吴林[1] 刘燃 吴冬琴 刘奕清 WU Lin;LIU Ran;WU Dong-qin;LIU Yi-qing(Chongqing Key Laboratory of Economic Plant Biotechnology/Chongqing Engineering ResearchCenter for Special Plant Seedlings,Chongqing University of Arts and Sciences,Yongchuan Chongqing402168,China;Chongqing Fuyuan Agricultural Biotechnology Research Institute Co.LTD,YongchuanChongqing 402168,China;Chongqing Yongchuan Democratic Progressive School,Yongchuan Chongqing 402168,China;Institute of Horticulture plants,College of Horticulture and Gardening,YangtzeUniversity,Jingzhou Hubei 434025,China)

机构地区：[1]重庆文理学院经济植物生物技术重庆市重点实验室/重庆市特色植物种苗工程技术研究中心,重庆永川402168 [2]重庆市幅沅农业生物技术研究院有限公司,重庆永川402168 [3]重庆市永川民进学校,重庆永川402168 [4]长江大学园艺园林学院/园艺植物研究院,湖北荆州434025

出　　处：《西南大学学报（自然科学版）》2020年第3期35-42,共8页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(31701972);重庆市科委基础科学与前沿技术项目(cstc2017jcyjAX0233);重庆文理学院人才引进项目(R2016TZ04);重庆市园艺学重点学科项目(2017ZDXK017).

摘　　要：为筛选响应月季花朵衰老的RhRRs基因以及分析其表达特性,采用RT-PCR,RACE PCR以及MEGA等生物技术方法,检测了RhRRs基因在月季花朵不同开放阶段的表达谱,克隆了Rh39694基因全长,开展了进化树分析.结果表明,RU39694与RU12149的表达谱受月季花朵衰老显著抑制;其中RU39694基因全长1292 bp,开放阅读框(ORF)为738 bp,编码245个氨基酸,含有A类RRs蛋白特有的DDK基序,蛋白分子式为C1176 H1912 N 324 O 401 S12,分子量为27.39 kDa,等电点(pI)为4.88;RU39694蛋白与拟南芥ARR8,ARR9亲缘关系最近,被命名为RhRR9;此外,RhRR9的表达谱受外源细胞分裂素(6-BA)处理显著诱导,而被脱落酸(ABA)与乙烯(C 2H 4)处理显著抑制,推测可能在植物激素(细胞分裂素、脱落酸、乙烯)拮抗调控月季花朵衰老中发挥重要功能.In order to screen RhRRs in response to rose flower senescence and to analyze their expression patterns,RT-PCR was used to monitor expression profiles of RhRRs in rose flower at different flowering stages and after different plant growth regulators treatments.Addtionally,RACE-PCR and MEGA were performed to clone the full length of Rh39694 and to analyze phylogenetic tree,respectively.The results showed that among four RhRRs,the transcripts of both RU39694 and RU12149 were significantly repressed during rose flower senescence.The full length of RU39694 was1292 bp,including an open reading frame of 738 bp.It encoded a protein of 245 amino acids with a typical DDK motif,which was conserved in Type-A RRs family.The formula of this protein was C1176 H1912 N 324 O 401 S 12 with a molecular weight of 27.39 kDa and a theoretical pI of 4.88.Phylogenetic tree analysis suggested that RU39694 shared high similarity with Arabidopsis ARR8 and ARR9,and was designated RhRR9.Additionally,the expression of RhRR9 was significantly induced by 6-BA treatment,while was strongly repressed by ABA or ethylene treatments.Taken together,these results supported an hypothesis that RhRR9 may play a critical role in regulation of rose flower senescence via antagonism of cytokinin to ABA and ethylene.

关 键 词：月季 响应调节因子 细胞分裂素 花朵衰老 

分 类 号：S685.12[农业科学—观赏园艺] Q945.48[农业科学—园艺学]