生物滴滤器处理间二氯苯废气的性能分析  

Performance analysis of m-dichlorobenzene waste gas treatment by biotrickling filter

作　　者：李悦 许琦 杨百忍[2] 丁成[2] 李朝霞[2] 王丽萍[3] 杨帅 顾於凡 LI Yue;XU Qi;YANG Bairen;DING Cheng;LI Zhaoxia;WANG Liping;YANG Shuai;GU Yufan(School of Environmental and Safety Engineering,Changzhou University,Changzhou 213164,China;School of Environmental Science and Engineering,Yancheng Institute of Technology,Yancheng 224051,China;School of Environment Science and Spatial Informatics,China University of Mining and Technology,Xuzhou 221116,China)

机构地区：[1]常州大学环境与安全工程学院,常州213164 [2]盐城工学院环境科学与工程学院,盐城224051 [3]中国矿业大学环境与测绘学院,徐州221116

出　　处：《环境工程学报》2020年第2期448-456,共9页Chinese Journal of Environmental Engineering

基　　金：国家重点研发计划(2016YFC0209200);国家自然科学基金资助项目(51778612);江苏省高校自然科学基金资助项目(BK20191480)。

摘　　要：从土壤中筛选1株能够降解间二氯苯能力的菌株,鉴定为土壤短芽孢杆菌(Brevibacillus agri),该优势菌最佳生长条件为:降解时间48 h,菌液接种量10%,pH=7,温度25℃。以间二氯苯为模拟有机废气,采用生物滴滤器接种土壤短芽孢杆菌的方法对其进行生物处理。结果表明,在空床停留时间为90 s、进气浓度为1 000 mg·m^-3、进气负荷为60 g·(m^3·h)^-1条件下,间二氯苯的去除率可以维持在85%以上。生物滴滤器稳定运行后,菌体表面官能团发生改变,通过傅里叶红外光谱和X射线光电子能谱分析发现,菌株通过逐步加氧羧化后开环降解间二氯苯;对菌株再进行16S rRNA基因序列的同源性分析发现,生物膜中Brevibacillus agri占比达69.39%,并可以良好生长。研究可为间二氯苯的工业化处理提供参考。In this study, a strain with m-dichlorobenzene degradability was screened from the soil and identified as Brevibacillus agri. The experimental results showed that the optimal growth conditions of the dominant bacteria were following: degradation time of 48 h, inoculum percent of 10%, pH=7, 25 ℃. Then mdichlorobenzene was used to simulate organic waste gas, which was treated by a biotrickling filter inoculated with Brevibacillus agri. The results showed when the empty bed residence time was 90 s, the intake gas concentration was 1 000 mg·m^-3, and the intake load rate was 60 g·(m^3·h)^-1, the removal rate of mdichlorobenzene could be maintained above 85%. After the biotrickling filter was stably operated, the functional groups on the surface of the cells were changed. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy indicated that the degradation of m-dichlorobenzene was through the oxygenationcarboxylation-ring-opening pathway. 16 S rRNA gene cloning and phylogenetic analysis performed on the strain showed that the proportion of Brevibacillus agri was up to 69.39%, and it presented a good growth in the biotrickling filter. This study can provide reference for the industrial scale treatment of dichlorobenzene.

关 键 词：间二氯苯 土壤短芽孢杆菌 官能团 生物滴滤器 生物处理 

分 类 号：X701[环境科学与工程—环境工程]