坦布苏病毒Du/CH/LSD/110128株原代毒及其鸡胚传代致弱毒在不同物种细胞中毒性和增殖能力的检测  

Detection of cytotoxicity and proliferative ability of Tembusu virus Du/CH/LSD/110128 strain and chicken-embryo passage attenuated virus in different species cells

作　　者：梁雨萌 马勇[1] 陈志杰 吴寒光 刘胜旺[1] 李海[1] LIANG Yumeng;MA Yong;CHEN Zhijie;WU Hanguang;LIU Shengwang;LI Hai(Division of Avian Respiratory Infectious Diseases/State Key laboratory of Veterinary Biotechnology of Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China)

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽呼吸道传染病创新团队,哈尔滨150069

出　　处：《黑龙江畜牧兽医》2020年第5期15-19,25,148,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：哈尔滨市科技创新人才研究专项(2016RAXYJ072)。

摘　　要：为了寻找坦布苏病毒(Tembusu virus,TMUV)致病机制的研究模型,试验利用实时荧光定量PCR技术检测坦布苏病毒Du/CH/LSD/110128株原代毒CF1及其鸡胚传代致弱毒CF100在不同物种细胞中的增殖情况,并将CF1与CF100分别在鸭胚中传代以检测病毒对鸭胚的损伤情况。结果表明:CF1可在禽类细胞、哺乳类细胞中增殖,但不引起细胞损伤,CF100在禽类细胞、哺乳类细胞中不能增殖,只能在Vero细胞中增殖,且不引起细胞损伤;CF1和CF100在鸭胚中传代发现,传代CF1的鸭胚全身出现明显的出血,但传代CF100的鸭胚未见出血。说明相对于原代毒CF1,鸡胚传代毒CF100对细胞、鸭胚的损伤均降低,具有一定的安全性,可作为坦布苏病毒致病机制的研究模型。In order to find a model for studying the pathogenic mechanism of Tembusu virus(TMUV),real-time fluorescent quantitative PCR was used to detect the proliferation of Du/CH/LSD/110128 CF1 and its chicken embryo passage virus CF100 in different species cells.CF1 and CF100 were passaged in duck embryos to detect the damage of the virus to duck embryos.The results showed that CF1 could proliferate in poultry and mammalian cells,but did not cause cell damage.CF100 could not proliferate in poultry and human cells,but only in Vero cells,and did not cause cell damage.CF1 and CF100 were passaged in duck embryos,and there was significant bleeding in the whole body of duck embryos passaged from CF1,but no bleeding was observed in duck embryos passaged from CF100.It indicated that CF100 had less damage to cells and duck embryos and had certain safety compared with CF1,and it can be used as a research model for the pathogenic mechanism of TMUV.

关 键 词：坦布苏病毒(Tembusu virus TMUV) 病毒感染 实时荧光定量PCR 病毒增殖 致细胞病变效应 研究模型 

分 类 号：S852.659.6[农业科学—基础兽医学]