溶血曼氏杆菌对药物及消毒剂的敏感性分析  

Sensitivity analysis of drugs and disinfectants for Mannheimia haemolytica

作　　者：韩小丽[1] 任静静[1] 杨铭伟[1] 朱玲[1] 张锐[1] 剡根强[1] HAN Xiaoli;REN Jinking;YANG Mingwei;ZHU Ling;ZHANG Rui;YAN Genqiang(College of Animal Science and Technology,Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832000

出　　处：《黑龙江畜牧兽医》2020年第5期112-114,124,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家科技支撑计划项目(2012BAD43B02)。

摘　　要：为了对引起肉牛运输热的病原溶血曼氏杆菌进行药物及消毒剂敏感性分析,试验对7株溶血曼氏杆菌进行19种药物的药敏试验,观察各药物抑菌圈直径并分析多重耐药性;选择3株菌株进行消毒剂消毒效果试验,在不同消毒剂不同浓度下,作用不同时间计算消毒剂的灭菌率。结果表明:7株菌株均对氨苄西林、环丙沙星、氟苯尼考、头孢拉定、头孢西丁、头孢噻肟、头孢噻吩、新霉素、万古霉素、替米考星等大部分药物敏感,对链霉素、四环素、阿莫西林、卡那霉素、土霉素青霉素、磺胺嘧啶钠耐药;3株菌在0.005%的次氯酸钠中作用10 min、在0.05%的氢氧化钠中作用20 min、在0.001%的碘液中作用15 min、在0.001%的过氧乙酸中作用10 min、在0.5%的甲醛中作用15 min均可被完全杀灭。说明溶血曼氏杆菌对大部分药物敏感,常用的消毒剂作用足够时间均可完全杀灭溶血曼氏杆菌。In order to analyze the susceptibility of drugs and disinfectants to the pathogens causing beef cattle shipping fever by Mannheimia haemolytica.The drug sensitivity test of 19 drugs was carried out on 7 strains of Mannheimia haemolytica,the diameter of bacteriostatic circle of each drug was observed and the multiple drug resistance was analyzed.The results showed that all 7 strains were sensitive to most drugs such as ampicillin,ciprofloxacin,florfenicol,cefradine,cefoxitin,cefotaxime,cefotaxime,neomycin,vancomycin,and tilmicosin.The three strains(Mh3,Mh4,Mh5)were killed completely in 0.005%sodium hypochlorite for 10 min,0.05%sodium hydroxide for 20 min,0.001%iodine solution for 15 min,0.001%peracetic acid for 10 min and 0.5%formaldehyde for 15 min.It indicated that Mannheimia haemolytica was sensitive to most of the drugs,and the commonly used disinfectant can kill it completely in enough time.

关 键 词：肉牛 运输热 溶血性曼氏杆菌 药敏试验 消毒剂敏感性 

分 类 号：S852.612[农业科学—基础兽医学]