不同产地、不同种属黄精的红外光谱鉴别研究  

Identification of Flavin from Different Habitats and Species by Infrared Spectroscopy

作　　者：高韵 司雨柔 王元媛 解玫莹 庄新港 程旺兴 GAO Yun;SI Yu-rou;WANG Yuan-yuan;XIE Mei-ying;ZHUANG Xin-gang;CHENG Wang-xing((1.Anhui Engineering Technology Research Center of Modern Chinese,School of Pharmacy and Medicine,Anhui University of Chinese Medicine,Hefei 230012,China;The 41st Research In-stitute of CETC,Bengbu 233010,China)

机构地区：[1]安徽中医药大学药学院现代中药安徽省工程技术研究中心,安徽合肥230012 [2]中国电子科技集团公司第四十一研究所,安徽蚌埠233010

出　　处：《化学试剂》2020年第3期275-279,共5页Chemical Reagents

基　　金：安徽高校自然科学研究项目(KJ2018A0300);安徽省重点研究与开发计划项目(201904a07020073);国家重点研发计划项目(2017YFC1701601)。

摘　　要：采用傅里叶红外光谱技术结合二阶导数光谱对不同产地的黄精进行鉴别。研究结果表明,不同产地多花黄精的红外光谱图在整体上比较相似,其主要吸收峰均在3270、2933、1623、1411、1372、1319、1269、1103、1019、926、865、815 cm-1附近,但吸收峰的位置、峰强度等方面存在明显差异,在1018 cm-1附近是糖类的特征吸收峰,由红外光谱图可推测安徽省池州市、岳西县、江西省宜春市多花黄精中糖类成分含量较高,湖北省黄冈市、安徽省黄山区、石台县次之,安徽省休宁县、金寨县相对较少。6种黄精属植物的红外光谱图在吸收峰的位置、形状、强度等方面均有差异,故可推测黄精属不同植物在化学成分及含量上均存在明显差异。二阶导数图呈现出许多原始图谱中被掩盖的谱峰的变化特征,将谱图间的差异更为明显得表现出来,进一步提高了谱图的分辨率,与普通红外光谱图相比,二阶导数红外光谱图进一步直观地验证了上述结果。Fourier transform infrared spectroscopy(FT-TR) and second derivative infrared spectroscopy are used to investigate Polygonatum cyrtonema Hua.The main absorption peaks were in the vicinity of 3 270,2 933,1 623,1 411,1 372,1 319,1 269,1 103,1 019,926,865 and 815 cm-1.The FT-IR spectra of Polygonatum cyrtonema Hua from different origins were similar,but the intensity and position of the absorption peaks were different.The absorption peak near 1 018 cm-1 was the characteristic absorption peak of glucose.From the infrared spectrum,it can be inferred that the content of glucose components in Chizhou,Yuexi,and Yichun were higher.Huanggang,Huangshan,Shitai were less.Xiuning and Jinzhai were relatively the least.The infrared spectra of six species of Polygonatum Mill were different in the position,shape and intensity of absorption peaks,so it can be inferred that there were obvious differences in chemical composition and content among different plants of Polygonatum Mill.The second derivative spectrum can spectrally strip the overlapping peaks in the original spectrum,further improving the resolution of the spectrum.Compared with the ordinary infrared spectra spectrum,the second derivative infrared spectrum further intuitively verified the above results.

关 键 词：多花黄精 不同产地 不同种属 鉴别 傅里叶红外光谱法 二阶导数光谱 

分 类 号：O657.33[理学—分析化学]