血液样本灭活处理对三种SARS-CoV-2抗体检测方法结果的影响  

Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies

作　　者：薛雄燕[1] 朱嫦琳[1] 黄少珍[1] 潘练华[1] 徐建华 李炜煊[1] XUE Xiongyan;ZHU Changlin;HUANG Shaozhen;PAN Lianhua;XU Jianhua;LI Weixuan(Clinical Laboratory,Foshan First People's Hospital,Foshan 528000,China;Medical Laboratory Center,Shunde Hospital of Guangzhou University of Chinese Medicine,Foshan 528333,China)

机构地区：[1]佛山市第一人民医院检验科,广东佛山528000 [2]广州中医药大学顺德医院医学检验中心,广东佛山528333

出　　处：《南方医科大学学报》2020年第3期316-320,共5页Journal of Southern Medical University

基　　金：佛山新型冠状病毒感染的肺炎应急攻关专项(2020001000431)。

摘　　要：目的探讨56℃30 min灭活处理对不同方法测定2019新型冠状病毒(SARS-CoV-2)抗体检测结果的影响。方法本文为回顾性研究。收集2020年2月12~18日在佛山市第一人民医院确诊为新型冠状病毒性肺炎患者血清样本、血浆样本及全血样本各11份,同时收集同期疑似病例及其他疾病患者血清、血浆及全血样本各10份,采用双盲法,在样本采用56℃30 min灭活处理前后,分别采用免疫层析法检测样本中的SARS-CoV-2总抗体、采用荧光免疫层析法检测样本中的SARS-CoV-2 IgM抗体、采用化学发光荧光免疫分析法检测血清及血浆样本SARS-CoV-2 IgM及SARS-CoV-2 IgG,比较灭活前与灭活处理后样本的抗体检测结果,并采用Pearson相关性分析灭活前后检测IgM及IgG半定量检测结果的相关性。结果免疫层析法检测灭活前后血清与血浆样本中SARS-CoV-2总抗体的阳性样本符合率为90.9%,阴性符合率为100.0%,总符合率均为95.2%。免疫层析法检测灭活前后全血样本中SARS-CoV-2总抗体的总符合率为100.0%。荧光免疫层析法检测灭活前后血清、血浆及全血样本中SARS-CoV-2 IgM抗体的阳性符合率均为100.0%,阴性符合率均为0%,总符合率均为47.6%。化学发光免疫分析法检测灭活前后血清样本中SARS-CoV-2 IgM及IgG抗体及血浆中的IgG抗体,阳性符合率、阴性符合率及总符合率均为100.0%,检测血浆IgM抗体的总符合率为95.2%。采用Pearson相关对灭活前后血清及血浆样本IgM和IgG半定量检测结果进行相关性分析,其中IgM灭活前后结果的相关系数为0.9999(95%CI:0.9998~1.000,P<0.001),IgG灭活前后结果的相关系数为0.9999(95%CI:0.9998~1.000,P<0.001)。结论 56℃30 min灭活处理血液样本对免疫层析法及化学发光免疫分析法检测SARS-CoV-2抗体结果几乎无影响,可在检测前先进行灭活以降低检验人员感染风险,荧光免疫层析法不可使用灭活后样本进行检测。Objective To evaluate the effects of heat inactivation of blood samples at 56 ℃ for 30 min on the results of SARS-CoV-2 antibody detection using different methods. Methods This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID-19 in our hospital. We collected samples of serum,plasma and whole blood from each patient between February, 12 and 18, 2020, and with a double-blind design, the samples were examined for SARS-CoV-2 antibodies before and after heat inactivation at 56 ℃ for 30 min. In all the samples, the total SARS-CoV-2 antibodies were detected using immunochromatography, and the IgM antibodies were detected using fluorescence immunochromatography;the IgM and IgG antibodies in the serum and plasma samples detected with chemiluminescence immunoassay. We compared the detection results and analyzed the correlation of semi-quantitative detection results of IgM and IgG antibodies before and after heat inactivation of the samples. Results With immunochromatography, the coincidence rate of the total SARS-CoV-2 antibody detection before and after heat inactivation of the serum and plasma samples was 90.0% in COVID-19 cases and 100.0% in the negative cases, resulting in a total coincidence rate95.2%;for the whole blood samples, the total coincidence rates of the total SARS-CoV-2 antibodies were 100.0%. For detection of IgM antibodies in the serum, plasma and whole blood samples using fluorescence immunochromatography, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were 100.0%, 0 and47.6%, respectively. For detection of serum IgM and IgG antibodies and plasma IgG antibodies with chemiluminescence immunoassay, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were all 100.0%, and the total coincidence rate of plasma IgM antibodies was 95.2%. Pearson correlation analysis of the se

关 键 词：2019新型冠状病毒 病毒灭活 抗体 血清 血浆 

分 类 号：R446.6[医药卫生—诊断学]