超声引导下小鼠心脏穿刺取血方法的可行性分析  

Feasibility of echocardiography-guided repeated intraventricular blood sampling in mice

作　　者：吴剑[1] 代方杰 游洁芸 丁志文 钱炳俊 黄家园[1] 徐冉 王晓燕[1] 苑洁[1] 邹云增[1] Wu Jian;Dai Fangjie;You Jieyun;Ding Zhiwen;Qian Bingjun;Huang Jiayuan;Xu Ran;Wang Xiaoyan;Yuan Jie;Zou Yunzeng(Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital and Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China;Department of Cardiology,Shanghai East Hospital,Tongji University,Shanghai 200120,China)

机构地区：[1]复旦大学附属中山医院/生物医学研究院,上海市心血管病研究所,200032 [2]上海市东方医院(同济大学附属东方医院)心内科,200120 [3]江苏医药职业学院医学技术学院,盐城224005

出　　处：《中华心血管病杂志》2020年第1期61-65,共5页Chinese Journal of Cardiology

基　　金：国家自然科学基金(31430039,81670228);上海市科委实验动物研究专项项目(16140901100);上海市卫计委科研课题面上项目(201640044);中山医院人才基金(No.014);中山医院优秀青年计划(2017ZSYXQN09)。

摘　　要：目的探索超声引导下不开胸进行小鼠心脏精准穿刺取血的方法,以明确该方法所能采集的最大血量,以及是否可以用于长期重复取血。方法将雄性C57BL/6J小鼠(10~14周龄)分为终止性实验组(n=4,检测一次所能取到的最大血量)、反复采血0.5 ml组(n=10,每次取0.5 ml全血,1次/2 d,持续4周),反复采血0.75 ml组(n=10,每次取0.75 ml全血,1次/2 d,持续4周)。使用高频心脏超声显示左心室最大切面,引导胰岛素注射器针头经胸廓进入左心室进行采血。在反复采血0.5 ml组,使用超声检测第一次采血前、第一次采血后3 min、以及采血结束(共采血14次)休息1周后的心脏结构和心功能变化。结果成功施行超声引导下不开胸经心脏穿刺取血,用时(88±19)s/只,获取的最大血量为(1.43±0.11)ml/只。在反复采血0.5 ml组,反复采血4周后,小鼠的心率、左心室射血分数、左心室缩短分数、左心室舒张末期内径及左心室舒张末期后壁厚度与第1次采血前比较,差异无统计学意义(P均>0.05),无小鼠死亡。但在反复采血0.75 ml组,在观察终点前有2只小鼠死亡。结论在超声引导下行小鼠心脏穿刺取血,安全、微创、便捷、高效,可获取血量大,也可用于长期多次取血。Objective To investigate the feasibility of echocardiography-guided closed-chest repeated intraventricular blood sampling in mice,and to clarify the maximum blood volume that can be collected by this method,and whether the method can be used for long-term repeated blood collection in mice.Methods Twenty-four male C57BL/6J mice(10-14 weeks old)were divided into the terminal experiment group(n=4,for investigating the maximum blood amount that could be sampled at one time),the repeated 0.5 ml blood collection group(n=10,sampling 0.5 ml whole blood each time,once every two days for consecutive 4 weeks),and the repeated 0.75 ml blood collection group(n=10,sampling 0.75 ml whole blood each time,once every two days for consecutive 4 weeks).High-frequency echocardiography was used to display the largest section of the left ventricle,guiding the insulin syringe needle through the thorax into the left ventricle for blood collection.In the repeated 0.5 ml blood collection group,echocardiography was used to detect the cardiac structure and function before blood collection,three minutes after blood collection,and one week after the last(the 14th)blood collection.Results We successfully performed echocardiography-guided closed-chest intraventricular blood sampling,with an average operating time(88±19)s per mouse,and a maximum blood volume(1.43±0.11)ml per mouse.In the repeated 0.5 ml blood collection group,heart rate,left ventricular ejection fraction,left ventricular fractional shortening,left ventricular end-diastolic dimension and left ventricular posterior wall end-diastolic thickness remained uncganged before the first blood collection and after 4 weeks of repeated blood collection(all P>0.05).No death in the repeated 0.5 ml blood collection group.However,in the 0.75 ml blood collection group,two mice died before the end point.Conclusions The echocardiography-guided closed-chest intraventricular blood sampling is a safe,minimally invasive,convenient and efficient method,and can be used repeatedly for long-term blood col

关 键 词：小鼠 超声心动描记术 多普勒 闭胸 穿刺 采血 

分 类 号：R54[医药卫生—心血管疾病] R44[医药卫生—内科学]