西维来司钠对烟雾吸入性肺损伤大鼠早期炎症反应、肺水肿发生率的影响  

Effect of sivelestat sodium on early inflammatory reaction and pulmonary edema in rats with smoke inhalation induced lung injury

作　　者：刘雪兰[1] 王明霞[1] Liu Xuelan;Wang Mingxia(Department of Emergency,Ningbo Medical Central Lihuili Hospital,Ningbo 315040,China)

机构地区：[1]宁波市医疗中心李惠利医院急诊科,315040

出　　处：《中国医师杂志》2020年第3期342-346,共5页Journal of Chinese Physician

基　　金：浙江省医药卫生科技计划(2018KY707)。

摘　　要：目的探讨西维来司钠对烟雾吸入性肺损伤大鼠早期炎症反应、肺水肿发生率的影响。方法选取75只SD大鼠,随机分为对照组、模型组、低剂量组、中剂量组及高剂量组,每组15只。除对照组外对其他大鼠进行烟雾吸入建模,并在建模后分别给予生理盐水、西维来司钠10 mg/kg、20 mg/kg及30 mg/kg腹腔注射,24 h后观察其一般情况及肺组织情况。结果与对照组大鼠相比,各组大鼠均存在一定程度的肺损伤、肺泡间壁增厚、肺泡间质充血等情况,且模型组大鼠肺泡间隔厚度、肺组织湿干比重(W/D)水平、肺水肿发生率及TNF-α、IL-6、中性粒细胞弹性蛋白酶(NE)、髓过氧化物酶(MPO)水平均显著高于其他4组,高、中、低剂量组中,以低剂量组大鼠肺功能恢复水平最高,其肺泡间隔厚度、肺组织W/D水平、肺水肿发生率分别为(6.76±0.40)μm、(5.18±0.13)及40.00%,其TNF-α、IL-6、NE及MPO水平分别为(19.62±0.83)pg/L、(9.41±0.25)pg/L、(6.23±0.18)μg/L及(30.64±0.87)pg/L,显著优于中剂量组及高剂量组(P<0.05)。结论西维来司钠对烟雾吸入性肺损伤能够产生显著干预效果,可提升其肺功能恢复水平,降低肺脏出现的炎症反应及肺水肿等症状发生,其治疗效果与给药剂量存在关联,就大鼠而言,给药剂量为10 mg/kg治疗效果最佳。Objective To investigate the effects of sivelestat sodium on early inflammatory response and pulmonary edema in rats with smoke inhalation-induced lung injury.Methods Seventy-five SpragueDawley rats were randomly divided into control group,model group,low-dose group,middle-dose group and high-dose group.The smoke inhalation model was continued except for the control group.The rats were given intraperitoneal injection of normal saline,cevicell sodium 10 mg/kg,20 mg/kg and 30 mg/kg.The general condition and lung tissue were observed after 24 hours.Results Compared with the control group,rats in each group had a certain degree of lung injury,alveolar wall thickening,alveolar interstitial hyperemia,etc.The alveolar septal thickness,lung tissue wet-dry(W/D)level,the incidence of edema and the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),neutrophil elastase(NE)and myeloperoxidase(MPO)in the model group were significantly higher than those of the other 4 groups.The lung function recovery was highest in the low dose group and the alveolar septal thickness was the highest among the dose group.The alveolar wall thickening incidence of W/D level and pulmonary edema in lung tissue in the low dose group were(6.76±0.40)μm,(5.18±0.13)and 40.00%,respectively,and the levels of TNF-α,IL-6,NE and MPO were(19.62±0.83)pg/L,(9.41±0.25)pg/L,(6.23±0.18)μg/L and(30.64±0.87)pg/L,significantly better than the middle dose group and high dose group(P<0.05).Conclusions Sivelestat sodium can significantly affect the smoke inhalation lung injury,and improve the recovery of liver function,which can significantly reduce the incidence of inflammation and pulmonary edema in the lung,but its therapeutic effect and drug delivery.There was a significant association between doses and therapeutic effect,in the case of rats,a dose of 10 mg/kg was the best for treatment.

关 键 词：肺损伤 烟雾吸入损伤 西维来司钠 炎症 肺水肿 大鼠 

分 类 号：R563[医药卫生—呼吸系统]