基于氮掺杂碳纳米颗粒荧光猝灭-恢复方法检测还原型谷胱甘肽  

Nitrogen-doped Carbon Nanoparticle Modulated Turn-on Fluorescent Probes for Glutathione Detection

作　　者：刘辉 赵永福 韩怀远 刘亚雯 冶保献[2] LIU Hui;ZHAO Yong-fu;HAN Huai-yuan;LIU Ya-wen;YE Bao-xian(College of Chemistry and Chemical Engineering,Zhengzhou Normal University,Zhengzhou 450044,China;College of Chemistry and Molecular Engineering,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]郑州师范学院化学化工学院,河南郑州450044 [2]郑州大学化学与分子工程学院,河南郑州450001

出　　处：《化学试剂》2020年第2期126-130,共5页Chemical Reagents

基　　金：河南省高等学校重点科研计划项目(20A-150008);郑州师范学院大学生创新性实验计划项目(DCY2017028);郑州师范学院分析化学课程教学团队项目(901029);郑州师范学院环境催化科研创新团队项目(702010)。

摘　　要：分别以海藻酸钠和色氨酸为碳源和氮源,采用固相法一步合成出了量子产率为47.9%的氮掺杂荧光碳纳米颗粒(N-CNPs)。根据铜离子存在的条件下,N-CNPs荧光强度的恢复情况与还原型谷胱甘肽浓度成正比的关系,建立基于N-CNPs检测还原型谷胱甘肽的新方法。优化了溶液pH及反应时间等条件。在pH 6.0、铜离子浓度30μmol/L条件下,谷胱甘肽在0.2~45μmol/L浓度范围内与N-CNPs荧光恢复强度呈良好的线性关系,检出限为50 nmol/L。方法具有灵敏度高、选择性好、操作简单等优点,可用于实际样品中谷胱甘肽的检测。Highly photo luminescent nitrogen-doped carbon nanoparticles(N-CNPs,fluorescent quantum yield of 47.9%) were prepared by a solid-phase synthesis strategy employing sodium alginate as a carbon source and tryptophan as nitrogen.Based on the relationship between the fluorescence intensity of N-CNPs/Cu2+ and the concentration of reduced glutathione,a novel assay for reduced glutathione was established.Under the optimized conditions,a linear relationship between the fluorescence recovery intensity of N-CNPs and the concentration of reduced glutathione was established in the range of 0.2~45 μmol/L.The detection limit was 50 nmol/L.The method has the advantages of high sensitivity,good selectivity and simple operation,and can be used for detecting glutathione in real samples.

关 键 词：荧光碳纳米颗粒 氮掺杂 谷胱甘肽 海藻酸钠 色氨酸 

分 类 号：O657.3[理学—分析化学]