CHOP在慢性间歇低氧大鼠肺组织表达水平变化  

Change of CHOP expression level in lung tissue of chronic intermittent hypoxia rats

作　　者：张嘉宾 戈艳蕾 刘聪辉 付爱双 王红阳 朱晓颖 李真真 ZHANG Jia-bin;GE Yan-lei;LIU Cong-hui;FU Ai-shuang;WANG Hong-yang;ZHU Xiao-ying;LI Zhen-zhen(Department of Respiratory,The Affiliated Hospital of North China University of Technology,Tangshan Hebei 063000,China)

机构地区：[1]华北理工大学附属医院呼吸科,河北唐山063000

出　　处：《蚌埠医学院学报》2020年第2期178-180,共3页Journal of Bengbu Medical College

基　　金：河北省医学科学研究重点课题计划(20180759)。

摘　　要：目的:建立慢性间歇低氧大鼠模型,探讨内质网应激标志蛋白CHOP在慢性间歇低氧大鼠肺组织表达水平变化。方法:选取Wistar雄性大鼠48只随机分成慢性间歇低氧组(CIH组)和对照组(UC组),每组各自分成4个时间亚组,分别为自实验之日起1周、2周、3周、4周,每个时间亚组6只大鼠。采用HE染色法及免疫组织化学法,观察比较CIH组与UC组大鼠肺组织病理形态变化及CHOP蛋白的表达变化。结果:CIH组大鼠肺泡结构破坏,肺泡壁间隔增宽,可见间质水肿,少量炎性细胞浸润,部分肺泡融合;UC组肺组织未见明显病理形态改变。免疫组织化学法检测结果提示,CHOP蛋白的表达主要集中于肺泡上皮细胞,CIH组大鼠肺组织CHOP蛋白表达较UC组明显增多,且随低氧时间延长,CHOP表达呈逐渐增多趋势(P<0.01)。结论:CHOP在慢性间歇低氧大鼠肺组织中表达水平升高。Objective:To establish the rat model of chronic intermittent hypoxia,and detect the expression level of endoplasmic reticulum stress marker protein CHOP in lung tissue of chronic intermittent hypoxia rats.Methods:Forty-eight male Wistar rats were randomly divided into the chronic intermittent hypoxic(CIH)group and control(UC)group.Each group was subdivided into 4 subgroups,including 1 week,2 weeks,3 weeks and 4 weeks subgreups(6 rats in eech subgroup).The histopathological changes of lung tissue and expression level of CHOP protein in CIH group and UC group were analyzed using HE staining and immunohistochemistry.Results:In CIH group,the alveolar structure was destroyed,the alveolar wall septum widened,the interstitial edema was seen,a small amount of inflammatory cells infiltrated,and the partial alveolar fused.No obvious pathological change was found in the UC group.The results of immunohistochemistry showed that the expression level of CHOP protein in CIH group rats was significantly higher than that in UC group,and the CHOP expression increased with the duration of hypoxia(P<0.01).Conclusions:The expression of CHOP in lung tissue of chronic intermittent hypoxia rats increases.

关 键 词：慢性间歇低氧 内质网应激 CHOP 肺组织 

分 类 号：R766[医药卫生—耳鼻咽喉科]