安胎合剂定性定量方法优化研究  

Optimization of Qualitative and Quantitative Methods for Antai Mixture

作　　者：管敏[1] 陈晓斌[1] GUAN Min;CHEN Xiaobin(Affiliated Hospital of Nanjing University of Chinese Medcine,Nanjing,Jiangsu,China 210029)

机构地区：[1]南京中医药大学附属医院,江苏南京210029

出　　处：《中国药业》2020年第7期119-122,共4页China Pharmaceuticals

基　　金：江苏省中医院2018年度院级课题[Y18044]。

摘　　要：目的优化安胎合剂的定性定量方法。方法通过薄层色谱法鉴别处方中的山茱萸和续断。采用高效液相色谱梯度洗脱法同时测定方中芍药苷和川续断皂苷Ⅵ的含量,检测波长为0~21 min时230 nm,21~45 min时212 nm。结果山茱萸、续断的薄层鉴别图清晰,分离度好,阴性对照无干扰;芍药苷,川续断皂苷Ⅵ质量浓度分别在12.625~404μg/mL和62.6875~2006μg/mL范围内与峰面积线性关系良好。结论优化的薄层鉴别方法专属性强,结果清晰;2个成分的含量测定方法简便,重复性好。Objective To optimize the qualitative and quantitative methods of Antai Mixture.Methods Fructus corni and Radix dipsaci of the prescription were identified by thin layer chromatography(TLC).The content of paeoniflorin and saponin VI were determined simultaneously using high performance liquid chromatography(HPLC)with gradient elution method;the detection wavelengths were 0-21 min:230 nm;21-45 min:212 nm.Results The thin layer identification map of Fructus corni and Radix dipsaci was clear,the separation was good,and the negative control had no interference.The peak area values of paeoniflorin and saponin VI showed good linear relationship within the scope of 12.625-404μg/mL and 62.6875-2006μg/mL.Conclusion The preferred TLC methods are distinct and specific;the content determination method of the two ingredients is simple and reproducible.

关 键 词：安胎合剂 芍药苷 川续断皂苷Ⅵ 薄层色谱法 高效液相色谱法 

分 类 号：R932[医药卫生—生药学] R286.0[医药卫生—药学]