微生态制剂中环状芽孢杆菌的分离鉴定及消毒技术评价  

Isolation and Identification of Bacillus circulans in Micro-ecological Preparations and Evaluation of Disinfection Technology

作　　者：孔洁 李亭玉 李太元[1] 常惠芸[2] KONG Jie;LI Ting-yu;LI Tai-yuan;CHANG Hui-yun(College of Agriculture,Yanbian University,Yanji 133002,China;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)

机构地区：[1]延边大学农学院,吉林延吉133002 [2]中国农业科学院兰州兽医研究所,甘肃兰州730046

出　　处：《畜牧与饲料科学》2020年第2期31-36,共6页Animal Husbandry and Feed Science

基　　金：吉林省重点科技研发项目(20180201074NY)。

摘　　要：[目的]分离微生物发酵菌液中耐热的芽孢杆菌,并分析几种常用消毒技术对环状芽孢杆菌的效果评价。[方法]通过100℃热处理30 min的方法从微生物发酵菌液中筛选耐热菌株,采用观察菌落形态与培养性状、革兰染色、生化鉴定及分子生物学鉴定等技术对其进行鉴定,构建系统发育树进行基因序列比对分析,并采用热空气处理、紫外线照射、过氧乙酸消毒技术对其进行消毒处理。[结果]从微生物发酵菌液中分离筛选出1株耐热细菌,经鉴定该菌株为环状芽孢杆菌;比较不同消毒技术对微生态制剂中环状芽孢杆菌的消毒效果发现,120℃干热条件下处理菌液60 min,120℃湿热条件处理菌液10 min,紫外线(辐射强度达到110μW/cm2)照射时间超过140 min,过氧乙酸体积分数大于2.75%的情况下,均可有效抑制环状芽孢杆菌生长。[结论]从微生物发酵菌液中成功分离到环状芽孢杆菌,其消毒效果试验为后续安全生产微生态制剂、优化菌种发酵条件提供了参考数据。[Objective]To isolate the heat-resistant Bacillus strain from the fermentation liquid of microorganisms,and to evaluate the disinfective effect of several commonly used disinfection techniques on Bacillus circulans.[Method]The heat-resistant strain was screened from the fermentation liquid of microorganisms by heat treatment at 100℃for 30 minutes.The obtained heat-resistant strain was identified via colony morphology,cultural characteristics,Gram staining,biochemical confirmation and molecular biological identification.A phylogenetic tree was constructed for gene sequence alignment analysis.The disinfective effect of hot air treatment,ultraviolet irradiation and peracetic acid disinfection techniques on the isolated strain was assessed.[Result]One heat-resistant bacterial strain was isolated from the fermentation liquid of microorganisms,and it was identified as Bacillus circulans.By comparing the disinfective effect of different disinfection technologies on Bacillus circulans in micro-ecological preparations,it was found that when the bacterial solution was treated at 120℃for 60 minutes under the dry heat condition and treated at 120℃for 10 minutes under the humid heat condition,and when the ultraviolet irradiation time exceeded 140 minutes(radiation intensity reached 110μW/cm2)and the volume fraction of peroxyacetic acid was higher than 2.75%,the growth of Bacillus circulans was effectively inhibited.[Conclusion]The Bacillus circulans strain was successfully isolated from the fermentation liquid of microorganisms,and the disinfection effective test provided references for the subsequent safe production of micro-ecological preparations and optimization of the fermentation conditions of the bacteria.

关 键 词：环状芽孢杆菌 微生态制剂 细菌分离鉴定 消毒技术 

分 类 号：S816.3[农业科学—饲料科学]