紫外可见分光光度法测定丹眠颗粒中总皂苷的含量  

Determination of Total Saponins in Danmian Granules by UV Spectrophotometry

作　　者：蒲翔 谢宇 罗君 黄颖 周芳丽 麻朝朝 王亮 李新建 龙丽慧 张丽艳 PU Xiang;XIE Yu;LUO Jun;HUANG Ying;ZHOU Fangli;MA Zhaozhao;WANG Liang;LI Xinjian;LONG Lihui;ZHANG Liyan(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China;Guizhou Guangjitang Pharmaceutical Company Limited,Guiyang 550014,Guizhou,China;First Affiliated Hospital of Guizhou Universityof Traditional Chinese Medicine(TCM),Guiyang 550001,Guizhou,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]贵州广济堂药业有限公司,贵州贵阳550014 [3]贵州中医药大学第一附属医院,贵州贵阳550001

出　　处：《贵州中医药大学学报》2020年第2期35-37,共3页Journal of Guizhou University of Traditional Chinese Medicine

基　　金：贵州省国内一流建设学科(中药学),项目编号:GNYL[2017]008号;贵州省科技支撑计划项目,项目编号:黔科合支撑[2018]2772;贵州省科技厅贵阳中医学院第一附属医院联合基金项目,项目编号:黔科合LH字[2015]7801;国家中医药管理局中药特色技术传承人才项目,项目编号:国中医药办人教函[2018]204号;贵阳国家高新技术产业开发区管理委员会科技型中小企业技术创新基金项目,项目编号:GXCX2015-012。

摘　　要：目的:建立可见-紫外分光光度法检测丹眠颗粒总皂苷的含量。方法:采用岛津UV-2501PC可见-紫外分光光度计,检测波长为560 nm。以人参皂苷Re为对照品,经显色测定吸光度,绘制标准曲线计算含量。结果:总皂苷在0.04~0.20μg/mL范围内线性关系良好,回归方程为Y=3.6673 x-0.0222,r=0.9990;精密度、重复性、稳定性试验RSD均小于2.0%;平均回收率为98.46%(RSD为1.56%,N=6)。结论:本实验方法简便准确、重复性好、灵敏度高、含量测定方法稳定可行,适用于丹眠颗粒中总皂苷的含量测定,可更好控制其质量。Objective:The UV spectrophotometry was established to detect the total saponin content of Danmian granules.Methods:Using Shimadzu UV-2501PC visible-ultraviolet spectrophotometer,the detection wavelength is 560 nm.Ginseng saponins Re was used as reference substance to determine absorbance by color rendering standard curve to calculate content.Results:A good linear relationship of total saponin was in the range of 0.04~0.20μg/mL.The regression equation was Y=3.6673x-0.0222,r=0.9990.The RSD values of precision,repeatability and stability test were less than 2.0%.The average recovery was 98.46%(RSD is 1.56%,N=6).Conclusion:The method is simple,accurate,reproducible,sensitive,and the method is stable and feasible.It is suitable for determination the content of total saponin in Danmian granules,which is better for control its quality.

关 键 词：丹眠颗粒 总皂苷 可见-紫外分光光度法 含量测定 

分 类 号：R286.0[医药卫生—中药学]