基于液滴微流控技术的细胞封装  

Cell Encapsulation Based on Droplet Microfluidic Technology

作　　者：周向东[1] 付超鹏 葛丹 姚冰鹏 唐浩东[1] 张浩[1] ZHOU Xiangdong;FU Chaopeng;GE Dan;YAO Bingpeng;TANG Haodong;ZHANG Hao(College of Chemical Engineering,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江工业大学化学工程学院,浙江杭州310014

出　　处：《化学反应工程与工艺》2019年第2期174-182,共9页Chemical Reaction Engineering and Technology

基　　金：国家自然科学基金青年基金(NSFC-21404090);浙江省自然科学基金青年基金(ZJNSF-LQ14B050001)。

摘　　要：液滴微流控技术是在微米尺度管道中精确操控互不相溶的液体发生乳化,从而快速稳定地产生结构可控、尺寸均匀液滴的技术。相较于传统液滴乳化的技术,液滴微流控为封装细胞提供了一种新颖的解决方案。本文综述了近年来在微流控芯片中封装细胞的研究进展,主要包括细胞在微液滴及水凝胶微球中的封装。同时简要总结了细胞负载微凝胶目前的应用,并展望了基于液滴微流控技术细胞封装的发展前景。Droplet-based microfluidics is a technology that precisely manipulates the immiscible fluids in micro-channel structures,so that droplets with well-controlled size and structures can be generated rapidly and consistently.In comparison to the conventional droplet-based cell encapsulation manners,the droplet-based microfluidics enabled novel solution in cell encapsulation.The recent advances in microfluidic-based encapsulation of cells in both droplets and hydrogel particles were reviewed.The current applications of cell-laden hydrogel particles were summarized and an outlook on future directions of the related fields was presented.

关 键 词：微流控 细胞封装 微液滴 微凝胶 

分 类 号：Q279[生物学—细胞生物学]