点击化学反应在蛋白质组学分析中的研究进展  

Applications of Click Chemistry Reaction for Proteomics Analysis

作　　者：占方玲 高思宇 谢元栋 张金铭[3] 李毅[2] 刘宁[1,3] ZHAN Fang-Ling;GAO Si-Yu;XIE Yuan-Dong;ZHANG Jin-Ming;LI Yi;LIU Ning(Central Laboratory,the Second Hospital of Jilin University,Changchun 130041,China;Stomatological Hospital of Jilin University,Changchun 130022,China;Key Laboratory of Zoonosis Research,Ministry of Education,Jilin University,Changchun 130061,China)

机构地区：[1]吉林大学第二医院中心实验室,长春130041 [2]吉林大学口腔医院,长春130022 [3]吉林大学人兽共患病研究教育部重点实验室,长春130061

出　　处：《分析化学》2020年第4期431-438,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.81472030);吉林省科技厅项目(No.20180101267JC);吉林大学研究生创新项目(No.101832018C070)资助。

摘　　要：一价铜催化的叠氮炔基的特异性反应通常被称为“点击化学反应”。点击化学反应作为一种重要的分子连接方式,能够在分子水平实现特定小分子基团之间的简单高效连接。因其快速高效、选择性高、条件温和、副反应少等优点,在蛋白质组学分析中有广泛应用,包括新生成蛋白的鉴定和多种翻译后修饰蛋白的鉴定等方面。因此,深入研究点击化学反应在蛋白质组学中的反应机制,对理解蛋白质的结构功能具有重要意义。本文综述了近年来点击化学反应在蛋白质组学中的几类重要应用,并对其未来的发展趋势进行了展望。Cu(I)-catalyzed azide-alkyne specific reaction,popularly known as the“click chemistry reaction”,is defined as an important molecular connection method,which can realize simple and efficient connection between specific small molecule groups at the molecular level.Because of its advantages such as high efficiency,high selectivity,mild conditions and few side reactions,click chemistry reaction has been widely used in proteomics analysis,including the identification of newly synthesized proteins and the identification of various post-translational modified proteins.Therefore,in-depth study of the reaction mechanism of click chemistry reaction in proteomics is very important for understanding the structure and function of proteins.The goal of this review is to highlight the progress of several important applications of click chemistry in proteomics analysis in recent years,and discuss the future trends of the click chemistry in proteomics.

关 键 词：点击化学反应 蛋白质组学 评述 

分 类 号：O621.251[理学—有机化学] Q51[理学—化学]