骨关节炎滑液的表面增强拉曼光谱技术研究  

Study on Synovial Fluid of Osteoarthritis by Surface-Enhanced Raman Scattering Spectroscopy

作　　者：朱勇康 马丹英 陆燕飞 尚林伟 赵远 张紫薇 尹建华[1] ZHU Yong-Kang;MA Dan-Ying;LU Yan-Fei;SHANG Lin-Wei;ZHAO Yuan;ZHANG Zi-Wei;YIN Jian-Hua(Department of Biomedical Engineering,Nanjing University of Aeronautics and Astronautics,Nanjing 211106,China)

机构地区：[1]南京航空航天大学生物医学工程系,南京211106

出　　处：《分析化学》2020年第4期484-490,共7页Chinese Journal of Analytical Chemistry

基　　金：江苏省六大人才高峰计划项目(No.SWYY-034);国家自然科学基金项目(No.61378087);南京航空航天大学研究生创新基地(实验室)开放基金项目(No.kfjj20180309)资助。

摘　　要：关节滑液(SF)作为关节重要组分,在关节运动、润滑、保护等方面起至关重要的作用。随着骨关节炎(OA)的发展,滑液内蛋白、多糖等成分也会发生改变,因此可作为OA检测的标志物。常规拉曼光谱法检测的灵敏度较低,难以实现对关节滑液内微/痕量成分的光谱学检测,因此,表面增强拉曼散射(SERS)技术成为微/痕量生物组织检测的优先选择。本研究首先利用配制的银纳米溶胶检测稀释血清和透明质酸(HA),以检验SERS技术用于关节滑液内含物检测的可行性,然后以患病早期(8周)OA犬类膝关节处滑液为实验对象进行SERS检测。结果表明,蛋白谱带和HA、硫酸多糖等多糖谱带的强度均明显增强,实现了关节滑液的微量检测分析。通过病变与健康关节滑液SERS光谱的对比分析发现,在OA早期,关节滑液中HA、硫酸多糖等多糖成分较健康滑液均呈现增加趋势,并与健康关节滑液中对应的相关谱带有显著性差异(p<0.05)。本研究将SERS技术用于关节滑液的检测,取得了较为理想的实验结果,为早期OA识别和病变规律的探究以及关节滑液等组织液的微/痕量检测开辟了新的路径。As an important part of joint,synovial fluid(SF)plays an important role in joint movement,lubrication and protection.The changes in main components of SF(proteins,proteoglycans,etc.)indicate osteoarthritis(OA)development,which may be used as biomarkers for OA diagnosis.Since detection sensitivity of Raman scattering is limited,it’s hard to realize micro/trace composition detection of SF.Due to high sensitivity and high specificity,surface-enhanced Raman scattering(SERS)has become a preferred option for micro/trace detection.In this study,diluted serum and hyaluronic acid(HA)were used to verify the SERS feasibility in SF detection by the self-made nano silver sol.The SF drawn from the knee joint with 8-week OA(early OA)was investigated by SERS technique,and it was found that the scattering intensities of the protein,polysaccharide and pyranose ring were obviously enhanced,which could be used for the micro detection of SF.By comparing SERS spectra of SFs of the early OA and the health,it was found that contents of polysaccharide HA,sulfated polysaccharide and pyranose increased in the OA,showing significant differences with those in the health(p<0.05).The method proposed here opened up a promising and novel way to early OA diagnosis and micro/trace detection of biological tissue fluids.

关 键 词：表面增强拉曼散射 关节滑液 多糖 骨关节炎 

分 类 号：O657.37[理学—分析化学] R684.3[理学—化学]