基于金纳米粒子/Fe-MIL-88-NH2的比率型C反应蛋白免疫传感器  

Ratio Type C-reactive Protein Immunosensor Based on Gold Nanoparticles/Fe-MIL-88-NH2

作　　者：邓燕 马玉婵 王敏 谷梦巧 迟宽能 胡蓉[1] 杨云慧[1] DENG Yan;MA Yu-Chan;WANG Min;GU Men-Qiao;CHI Kuan-Neng;HU Rong;YANG Yun-Hui(College of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学化学化工学院,昆明650500

出　　处：《分析化学》2020年第4期498-506,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21465026,21765026)资助。

摘　　要：以金纳米粒子(Aunanoparticles,AuNPs)功能化的金属有机骨架材料(Fe-MOFs,Fe-MIL-88-NH2)为基质材料,构建了双信号比率型免疫传感器,用于C-反应蛋白(C-reactiveprotein,CRP)的检测。八面体结构的Fe-MOFs不仅提供了较大的有效表面积,可增加固定生物分子的量,促进电子和离子的传输,而且表现出良好的导电性。将AuNPs修饰到Fe-MOFs上可以进一步增加比表面积,以捕获大量抗体以及提高电子转移能力。随CRP浓度的增加,K3Fe(CN)6/K4Fe(CN)6的氧化峰电流减小,而Fe-MOFs的氧化峰电流相对恒定。采用K3Fe(CN)6/K4Fe(CN)6响应电流与Fe-MOFs的响应电流比值作为定量测定CRP的响应信号。以K3Fe(CN)6/K4Fe(CN)6作为信号探针(K3Fe(CN)6/K4Fe(CN)6-sP),AuNPs修饰的Fe-MOFs作为内参比探针(Fe-MOFs-rP),这种比率探针将sP和rP整合到一个结构中,确保了完全相同的修饰条件以及sP和rP在同一个传感界面上的相互依赖性,因此具有更强的消除环境干扰的能力。所构建的免疫传感器线性响应范围为1~200ng/mL,检出限为0.3ng/mL(S/N=3)。此传感器具有良好的选择性,有望用于心血管疾病的早期筛查和诊断。本研究为制备检测其它生物标志物的比率信号放大传感器奠定了基础。A dual-signal proportional immunosensor for detection of C-reactive protein was developed using gold-nanoparticles(AuNPs)functionalized metal-organic frameworks(MOFs)material(AuNPs/Fe-MIL-88-NH2)as an immobilized matrix and signal unit.The octahedral Fe-MOFs can not only provide a larger effective surface area,but also increase the amount of immobilized biomolecules and promote the transport of electrons and ions,and also exhibit good electrical conductivity.Modification of AuNPs onto Fe-MOFs can further increase specific surface area to capture large amounts of antibodies and increase electron transfer capacity.With increase of CRP concentration,the oxidation peak current of K3Fe(CN)6/K 4Fe(CN)6 decreases,while the oxidation peak current in Fe-MOFs is relatively constant.The response current ratio of K 3Fe(CN)6/K4 Fe(CN)6 to Fe-MOFs has been used as a quantitative measurement of CRP response signal.Fe-MOFs modified by K 3Fe(CN)6/K 4Fe(CN)6 as a signal probe(K3Fe(CN)6/K 4Fe(CN)6-sP)and AuNPs as internal reference probes(Fe-MOFs-rP).This ratio probe integrates sP and rP into one structure,ensuring identical modification conditions and the interdependence of sP and rP on a sensing interface.Therefore,this ratiometric probe has a stronger ability to eliminate environmental variation interference.The linear response of the immunosensor proposed here ranges from 1 to 200 ng/mL,and the detection limit is 0.3 ng/mL(S/N=3).The sensor has good selectivity and is expected to be used for early screening and diagnosis of cardiovascular disease.The method also lays the foundation for the preparation of other ratiometric signal amplification sensors for detecting biomarkers.

关 键 词：C-反应蛋白 金属有机骨架材料 比率型电化学免疫传感器 

分 类 号：R446.1[医药卫生—诊断学] O657.1[医药卫生—临床医学]