两种人抗狂犬病病毒抗体检测方法的分析  

An evaluation on ELISA for the test of rabies virus antibody by RFFIT

作　　者：梁振山[1] 于海珍[1] 崔为国[1] 彭小婉[1] 李梦壇 王哲[1] LIANG Zhenshan;YU Haizhen;CUI Weiguo;PENG Xiaowan;LI Mengtan;WANG Zhe(Henan Center for Disease Control and Prevention,Zhengzhou,Henan 450016,China)

机构地区：[1]河南省疾病预防控制中心,河南郑州450016

出　　处：《河南预防医学杂志》2020年第4期251-253,共3页Henan Journal of Preventive Medicine

基　　金：2018年河南省科技攻关项目(182102310088)。

摘　　要：目的以快速荧光灶抑制试验(RFFIT)检测结果为金标准,评价间接性酶联免疫吸附试验(ELISA)检测人血清样品中抗狂犬病病毒抗体的效能。方法对80份接种过狂犬病疫苗的人血清样品分别采用RFFIT和间接性ELISA检测抗狂犬病病毒抗体,分析两种方法检测结果的相关性和一致性。结果经回归分析,两方法检测结果之间有线性关系,回归方程Y=4.622+13.103X,相关系数r=0.261;经χ^2检验两种方法阳性检出率差异无统计学意义(χ^2=2.25,P>0.05),且两种检测方法结果之间有相关性(χ^2=13.472,P<0.001);一致性检验Kappa值为0.4805。结论本研究评价的间接性ELISA与RFFIT检测结果之间有相关性,具有中度一致性,尚需进一步研究。Objective To determine the efficiency of ELISA on the detection of antibodies against rabies virus in hu-man sera compared with the results of RFFIT as gold standard.Methods 80 human sera are tested by RFFIT for rabies virus neutralizing antibodies and by ELISA for antibodies against rabies virus.The correlation and consistency between the results of ELISA and RFFIT was analysized by statistic methods.Results By logistics analysis,the results of ELISA and RFFIT were correlated,Y=4.622+13.103X,the correlative coefficient(r)was 0.261.Byχ^2 test,the difference be-tween the positive rate of ELISA and RFFIT results was not significant(χ^2=2.25,P>0.05),the qualitative results be-tween ELISA and RFFIT was related(χ^2=13.472,P<0.001).The consistency test Kappa was 0.4805.Conclusions The results of ELISA and RFFIT are correlated.The ELISA should be improved continuously.

关 键 词：酶联免疫吸附试验 快速荧光灶抑制试验 相关性 一致性 

分 类 号：R446.62[医药卫生—诊断学]