多舱室爆炸致比格犬血清皮质醇、生长激素、泌乳素的变化及其与伤情的关系  

Effects of multi-cabins explosion on serum levels of cortisol,GH and PRL and their relationship with injury severity in Beagle dogs

作　　者：皇甫罗锴 程岗 贾博 王淑为 郭胜利 任斌 李彦腾 张剑宁 HUANGFU Luo-kai;CHENG Gang;JIA Bo;WANG Shu-wei;GUO Sheng-li;REN Bin;LI Yan-teng(Department of Neurosurgery,The Sixth Medical Center,Chinese PLA General Hospital,Beijing 100048,China)

机构地区：[1]中国人民解放军总医院第六医学中心神经外科,北京100048

出　　处：《中国临床神经外科杂志》2020年第2期94-97,共4页Chinese Journal of Clinical Neurosurgery

基　　金：全军后勤科研重大项目(AHJl4J001)。

摘　　要：目的探讨比格犬多舱室爆震伤后血清快速反应激素皮质醇和非快速反应激素[生长激素(GH)、泌乳素(PRL)]的变化,及其与颅脑损伤程度的关系。方法建造与实际舰船等大小的相邻舱室,并取健康成年比格犬48只随机置于当舱和邻舱。海军用炮弹于当舱进行静爆,利用压力传感器记录舱室内压力变化情况并测定爆炸前及爆炸后0.5、3、18 h当舱与邻舱实验犬血清神经元特异性烯醇化酶(NSE)、皮质醇、GH和PRL的含量。结果当舱内压力在起爆后出现明显的单个波峰(压力>3 000kPa),远高于邻舱最高峰(压力<170 k Pa)。当舱实验犬总死亡率(70.83%)明显高于邻舱实验犬(12.50%;P<0.05)。当舱和邻舱实验犬血清NSE、GH、皮质醇在爆炸后均持续高于爆炸前(P<0.05),而血清PRL未出现明显波动(P>0.05)。伤后18 h,当舱实验犬血清GH水平明显高于邻舱实验犬(P<0.05)。结论当舱实验犬较邻舱实验犬遭受到更为严重的颅脑爆震伤,伤后超急性期血清GH的测定可能有助于伤情的判断。Objective To investigate the changes in the serum levels of cortisol,growth hormone(GH),prolactin(PRL)after the multi-cabins explosion and their relationship with injury severity in beagle dogs.Methods Twenty-four beagle dogs were placed respectively in the blast cabin and adjacent cabin which were similar to real cabins in warship.The models of multi-cabins explosion injury were established with a projectile.The serum levels of neuron specific enolase(NSE),cortisol,GH and PRL were determined in all the dogs before the explosion and 0.5,3 and 18 hours after the explosion.In the meanwhile,the pressure was determined by pressure sensor in all the cabins.Results The distinct single peak of pressure was significantly higher in blast cabin than that in the adjacent cabin(P<0.01)and the total mortality of the dogs(70.83%)was significantly higher in the blast cabin than that(12.5%)in the adjacent cabin(P<0.05).The serum levels of NSE,GH and cortisol were significant higher after the explosion than those before the explosion in these dogs(P<0.05).The serum levels of NSE and GH were significantly higher in the blast cabin group than those in the adjacent cabin group 18 hours after the explosion(P<0.05).The insignificant change in the serum level of PRL was observed after the explosion in all the dogs compared with that before the explosion(P>0.05).Conclusions Compared with the dogs in the adjacent cabin group,dogs in blast cabin GROUP suffer from more severe blast-traumatic brain injury.The detecting of serum GH levels in hyperacute phase may be helpful to the judgment of the injury severity.

关 键 词：颅脑爆震伤 多舱室爆震伤 血清水平 皮质醇 生长激素 泌乳素 比格犬 

分 类 号：R651.15[医药卫生—外科学]