基于栅藻延迟发光表征中药药性的方法学验证  

Methodological verification of characterization of traditional Chinese medicine based on delayed luminescence of scenedesmus

作　　者：闵令圆 庞靖祥[2] 杨美娜[2] 周宝宸 荆绮 韩金祥[2] MIN Lingyuan;PANG Jingxiang;YANG Meina;ZHOU Baochen;JING Qi;HAN Jinxiang(School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medical Sciences,Jinan 250020,China;Shandong Medicinal Biotechnology Center,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250062)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,济南250020 [2]山东省医药生物技术研究中心山东第一医科大学(山东省医学科学院),济南250062

出　　处：《生物医学工程研究》2020年第1期90-94,共5页Journal Of Biomedical Engineering Research

基　　金：国家国际科技合作专项资助项目(2014DFA30380);山东省重点研发计划项目(2019GSF108168,2019LYXZ037);山东省医学科学院院级科技计划项目(2018-61)。

摘　　要：对栅藻延迟发光定量化表征中药寒热药性的方法进行优化,并进行方法学验证。用YPMS-2生物光子测量仪检测栅藻激发延迟发光强度,对测得的激发延迟发光强度用Statistica 10.0软件进行线性拟合,得到表征栅藻性质的可靠参数K,用产自于道地产地的经典寒性中药黄芩作为样本,验证本研究方法的精密度、稳定性、重复性,并进行空白实验。栅藻最佳使用K值波动范围是2.81~3.89,本研究方法精密度高,重复性、稳定性良好,空白溶剂无干扰。The method of quantitatively characterizing the cold and hot medicinal properties of Chinese medicinal herbs was optimized for delayed luminescence,and methodological verification was carried out.The YPMS-2 biophoton measuring instrument was used to detect the delayed luminescence intensity of the S.cerevisiae.The measured excitation delay luminescence intensity was linearly fitted with Statistica 10.0 software to obtain a reliable parameter K for characterizing the properties of S.cerevisiae.The classical cold Chinese medicine Astragalus membranaceus was used as a sample to study the precision,stability,and repeatability of the method and to carry out blank experiments.The optimum K-value fluctuation range of Scenedesmus is 2.81~3.89.The method has high precision,good repeatability and stability,and no interference from blank solvent.

关 键 词：栅藻 方法学验证 生物光子 生物指示剂 中药药性 延迟发光 

分 类 号：R318[医药卫生—生物医学工程]