磷脂酶Dα1与H2S参与冬凌草甲素对拟南芥的化感作用  

Phospholipase Dα1 and hydrogen sulfide were involved in the allelopathy of oridonin to Arabidopsis thaliana

作　　者：赵启安 刘博 张玥 刘琴[1] 张莉环 刘瑞芳 杨宁[1,2] ZHAO Qi-an;LIU Bo;ZHANG Yue;LIU Qin;ZHANG Li-huan;LIU Rui-fang;YANG Ning(College of Life Sciences,Northwest Normal University,730070,Lanzhou,China;Key Laboratory of Cell Activities and Stress Adaptations,Ministry of Education,Lanzhou 730000,China)

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]细胞活动与逆境适应教育部重点实验室,兰州730000

出　　处：《应用生态学报》2020年第3期959-968,共10页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(31960061,31660116);“细胞活动与逆境适应教育部重点实验室开放基金”项目资助。

摘　　要：以拟南芥哥伦比亚野生型(WT)、磷脂酶Dα1(PLDα1)缺失型突变体pldα1、D-/L-半胱氨酸脱巯基酶(D-/L-CDes)缺失型突变体d-cdes和l-cdes幼苗为试验材料,60μmol·L^-1冬凌草甲素为处理浓度,研究了拟南芥响应二萜类化合物冬凌草甲素的化感作用中磷脂酶Dα1(PLDα1)与气体信号分子硫化氢(H2S)的信号关系。结果表明:冬凌草甲素显著提高了野生型拟南芥幼苗H2S含量、PLD和D-/L-CDes酶的活性及其基因表达;冬凌草甲素处理下,pldα1突变体幼苗的D-CDes和L-CDes活性明显低于WT,外源添加磷脂酸(PA)后D-CDes和L-CDes活性显著提高,并高于WT;冬凌草甲素显著抑制4种株系根的生长,其中d-cdes和l-cdes对冬凌草甲素更加敏感,外施NaHS可以促进冬凌草甲素处理下4种株系根的生长及内源H2S产生,外施PA只对冬凌草甲素处理下的WT、pldα1和l-cdes株系根的生长及内源H2S产生有促进作用,而对d-cdes株系没有明显作用。说明PLDα1和H2S在拟南芥响应冬凌草甲素过程中发挥作用,且PLDα1/PA位于D-CDes上游,参与调控拟南芥幼苗H2S的产生及根生长的信号过程。We investigated the signal relationship between phospholipase Dα1(PLDα1)and the gas signal molecule hydrogen sulfide(H2S)in Arabidopsis thaliana response to the allelopathy of diterpenoid oridonin.The wild type Arabidopsis Columbia(WT),phospholipase Dα1(PLDα1)deletion mutant pldα1,D-/L-cysteine desulfyrase synthetic deletion mutant d-cdes and l-cdes seedlings were used as experiment materials,while 60μmol·L^-1 oridonin was applied as treatment concentration.The results showed that oridonin significantly increased H2S content,PLD and D-/L-CDes activities,and gene expressions of PLDα1 and D-/L-CDes in WT.Under oridonin treatment,the D-CDes and L-CDes activities of pldɑ1 seedlings were significantly lower than those of WT.Both D-CDes and L-CDes activities increased after exogenous addition of phosphatidic acid(PA)and were higher than those of WT.Oridonin significantly inhibited root growth of four lines,with d-cdes and l-cdes being more sensitive to oridonin.Application of NaHS promoted root growth and endogenous H2S production of four lines under oridonin treatment,while application of PA increased root growth and endogenous H2S production in WT,pldɑ1 and l-cdes,but had no effect in d-cdes.These results indicated that PLDα1 and H2S played a vital role in driving the response of Arabidopsis to oridonin,and that PLDα1/PA was located at the upstream of D-CDes to participate the regulation of the H2S production and root growth.

关 键 词：冬凌草甲素 磷脂酶Dα1 硫化氢 化感作用 

分 类 号：Q945[生物学—植物学]