21份玉米单倍体诱导系遗传背景及诱导率分析  

Analysis of Genetic Background and Inducing Rate of 21 Haploid Inducers

作　　者：王薪淇 岳尧海[1] 董亚琳[1] 孟令聪[1] 路明[1] 周德龙 孙传波[1] WANG Xinqi;YUE Yaohai;DONG Yalin;MENG Lingcong;LU Ming;ZHOU Delong;SUN Chuanbo(Jilin Academy of Agricultural Sciences/National Engineering Laboratory For Maize(Changchun)/National Engi neering Research Center for Maize(Jilin)/Key Laboratory Biology and Genetic Improvement of Maize in Northeast Re gion,Ministry of Agriculture/Jilin Provincial Open Laboratory of Crop Breeding in Hainan Base,Changchun 130033,China)

机构地区：[1]吉林省农业科学院/玉米国家工程实验室(长春)/国家玉米工程技术研究中心(吉林)/农业部东北中部玉米生物学与遗传育种重点实验室/吉林省农作物育种南繁基地开放实验室,长春130033

出　　处：《东北农业科学》2019年第6期10-13,32,共5页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业科学院创新工程项目(CXGC82018ZY017、C92071513);吉林省农业科技创新工程项目-博士后基金(c92071510)。

摘　　要：为明确改良和应用不同血缘背景诱导系,本研究选用21个外引及自选诱导系,采用基于Geno Plexs多重PCR的1K SNP标记进行基因分型并应用层次聚类法进行类群划分,同时以先玉335和3份糯玉米杂交种为母本进行杂交诱导,用以评判诱导系的诱导能力。结果表明,21份诱导系被划分为3类血缘,分别是Stock6为代表的类群Ⅰ、吉诱101代表的类群Ⅱ和农大高诱系列代表的类群Ⅲ;类群Ⅱ平均诱导率显著高于类群Ⅲ和类群Ⅰ,自选系WY7在选育过程中相比于背景诱导系产生了诱导率增益,WY7和W7-4可用于诱导糯玉米。In order to improve and apply the haploid inducers of different blood backgrounds clearly, in this study,21 introduced and self-selective haploid inducers were selected, and Geno Plexs multiplex PCR-based 1 K SNPmarkers were used for genotyping and hierarchical clustering was used for group classification.’Xianyu 335’ and 3 waxy hybrids were used as a female parent to induce hybridization to judge the inducing ability of the haploid induc-ers. The results showed that 21 induction lines were classified into three types of blood, among which group Ⅰ repre-sented by Stock6, group Ⅱ represented by ’Jiyou 101’, and group Ⅲ represented by the CAUHO. The average in-ducing rate of group Ⅱ was significantly higher than group Ⅲ and group Ⅰ. The self-selected lines,’Jiyou 101’ andWY7 produced an induction rate gain compared to the background haploid inducers during the selection process.WY7 and W7-4 can be used to induce waxy corn.

关 键 词：诱导系 诱导率 聚类分析 吉诱101 

分 类 号：S513[农业科学—作物学]