水稻穂顶部颖花退化T-DNA插入突变体的分子鉴定及表型特征分析  

Molecular Characterization and Phenotype Analysis of Panicle Apical Abortion T-DNA Mutants in Rice

作　　者：朴日花[1] 金永梅[1] 金京花[1] 金国光[1] 赵亚东[1] Koh Hee-Jong 董英山[1] 张强[1] PIAO Rihua;JIN Yongmei;JIN Jinghua;JIN Guoguang;ZHAO Yadong;Koh Hee-Jong;DONG Yingshan;ZHANG Qiang(Jilin Academy of Agricultural Sciences,Changchun 130033,China;Department of Plant Science of Agriculture and Life Sciences,Seoul National University,Seoul 00826,Korea)

机构地区：[1]吉林省农业科学院,长春130033 [2]韩国首尔大学植物生产科学部,首尔00826

出　　处：《东北农业科学》2019年第6期27-32,共6页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业科技创新工程项目(CXGC2017TD009);中国农业科技东北创新中心博士后研究项目(C4207010309);吉林省农业科技创新工程人才基金(C6215000216)。

摘　　要：水稻穗部是重要的农艺性状之一,穂顶部颖花退化会导致水稻产量大幅下降。本研究对水稻穗退化T-DNA突变体进行分子鉴定和表型分析,旨在进一步探明水稻穗发育的调控机制。我们从水稻突变体库POSTECH RISD(Rice T-DNA Insertion Sequence Database)中筛选到了3个T-DNA插入突变体,paa1、paa2和paa3。这些突变体中T-DNA分别插入到水稻穂顶部颖花退化候选基因LOCOs04g56160的第1个内含子、第8个外显子和3′端非翻译区。分别在T-DNA插入位点两侧水稻基因组和T-DNA片段上共设计3条引物,利用PCR方法鉴定出paa1、paa2和paa3突变纯合体与杂合体。3个突变杂合体自交后代(T3)植株表型与基因型共分离分析结果显示,paa1及paa2植株的穗顶部颖花退化表型与基因型相吻合,而paa3植株的颖花发育正常。paa2与PAA-Hwa(LOCOs04g56160基因点突变体,表型为穗顶部颖花退化)杂交,F1代植株表现为穂顶部颖花退化。农艺性状调查结果表明,paa1及paa2与对照品种相比在株高、穗长、单株有效分蘖数等所有农艺性状上存在显著差异,然而paa3仅在部分农艺性状上表现差异。以上结果表明paa1和paa2为穗顶部颖花退化T-DNA插入突变体,LOCOs04g56160为水稻穗顶部颖花退化调控基因,该结果为水稻穗发育研究奠定了理论基础。The architecture of panicles is an important agronomic trait, that the panicle apical abortion can cause significant yield reduction in rice. In the present study, in order to better understand the regulation mechanism of rice panicle development, the molecular characterization and phenotype analysis of panicle apical abortion T-DNA mutants were performed. Three panicle apical abortion(paa) mutants, paa1, paa2 and paa3, were identified and characterized from POSTECH Rice T-DNA Insertion mutant population. T-DNA insertion sites of these mutants were on the 1st intron of paa1, 8th exon of paa2 and 3’-UTR of paa3 at the rice gene of LOCOs04 g56160, respectively.Three primers were designed on both sides of the T-DNA insertion site on LOCOs04 g56160 gene and T-DNA fragments, respectively, then homozygotes and heterozygotes of paa1, paa2 and paa3 were identified by PCR. The genotypic segregation of the paa1 and paa2 was tightly linked with the phenotypic segregation of panicle apical abortion(bald tip) in the T3 progeny of their heterozygotes, while the apical panicle development of paa3 was normal. All F1 progeny of the cross between paa1 and PAA-Hwa(panicle apical abortion mutant which point mutation of LOCOs04 g56160) displayed panicle apical abortion phenotype. Agronomic traits of the paa1 and paa2 homozygotes was significantly reduced compared to wild type such as plant height, spike length, number of tillers and spike number per panicle. However,paa3 was reduced only in plant height, spike length and spike number. All these results indicated that paa1 and paa2 were panicle apical abortion mutants, and LOCOs04 g56160 confers panicle apical abortion characters. This study laid a theoretical foundation for the research on rice spike development.

关 键 词：水稻 穂顶部颖花退化 T-DNA插入突变体 

分 类 号：S511[农业科学—作物学]