枯草芽胞杆菌GB519发酵液菌体数量和芽胞率检测方法的比较  

Methods Comparison for Detecting Sporular Amount and Sporulation Efficiency of Bacillus Subtilis GB519

作　　者：朱峰[1] 王继春[1] 田成丽 向媛媛 张富浩 祁山颜 王东元[3] 姜兆远[1] 刘晓梅[1] 李莉[1] 任金平[1] 窦忠玉[4] ZHU Feng;WANG Jichun;TIAN Chengli;XIANG Yuanyuan;ZHANG Fuhao;QI Shanyan;WANG Dongyuan;JIANG Zhaoyuan;LIU Xiaomei;LI Li;REN Jinping;DOU Zhongyu(Institute of Plant Protection/Key Laboratory of Integrated Pest Management on Crops in Northeast,Ministry of Agriculture,Jilin Academy of Agricultural Sciences,Gongzhuling 136100;College of Life Science&Technology,Harbin Normal University,Harbin 150025;Plant Protection College,Shenyang Agricultural University,Liaoning Shenyang 110866;Institute of Crop Resources,Jilin Academy of Agricultural Sciences,Gongzhuling 136100,Chi na)

机构地区：[1]吉林省农业科学院植物保护研究所/农业部东北作物有害生物综合治理重点实验室,吉林公主岭136100 [2]哈尔滨师范大学生命科学与技术学院,哈尔滨150025 [3]沈阳农业大学植物保护学院,沈阳110866 [4]吉林省农业科学院作物资源研究所,吉林公主岭136100

出　　处：《东北农业科学》2019年第6期49-52,共4页Journal of Northeast Agricultural Sciences

基　　金：国家重点研发计划项目(2018YFD0200200、2018YFD0300200);吉林省农业科技创新工程项目(CXGC2017TD010);吉林省农业科学院博士后基金项目(188320)。

摘　　要：为了提高枯草芽胞杆菌GB519发酵液菌体数量和芽胞率的统计效率,本研究采用平板涂布法和平板倾注法进行活菌计数,利用加热除菌法、结晶紫染色法和孔雀绿染色法开展芽胞率检测效率的比较研究。结果表明:平板涂布法与倾注法的菌体计数数量差异不大,误差率相对一致。枯草芽胞杆菌GB519利用结晶紫染色法与孔雀绿染色法检测芽胞率差异不显著。确定了枯草芽胞杆菌GB519加热除菌法菌体致死温度为80℃、时间为15 min,其芽胞量为131.9×10^8 CFU/mL,芽胞率为50.1%,与孔雀绿染色法(54.8%)差异不显著,与结晶紫染色法芽胞率(58.7%)差异显著。本研究将为类似的芽胞检测研究提供方法和数据参考。In order to improve the statistical efficiency of the sporular amount and germination rate in the fermentation broth of Bacillus subtilis GB519,the methods both plate coating and plate pouring were used to count the viable bacteria. And the methods of heat sterilization,crystal violet and malachite green staining were used to compare with the germination rate. The results showed that there was little difference in sporular amount between plate coating and pouring method,and the error rate was relatively consistent. It was determined that the lethal temperature and time of heat sterilization method were at 80℃ for 15 min,and the sporular amount and germination rate of that were 131.9×10^8 CFU/mL and 50.1%,respectively,which was no significantly different from malachite green staining(54.8%),and was significantly different from that of crystal violet staining(58.7%). This study could provide positive conferences from both parameters and methods for the similar research.

关 键 词：枯草芽胞杆菌GB519 活菌计数 芽胞 芽胞率 

分 类 号：Q93-3[生物学—微生物学]