吉林、黑龙江部分地区毛葱OYDV感染情况调查  

Investigation of OYDV Infection on Shallot in Part Areas of Jilin and Heilongjiang Province

作　　者：李小宇[1] 郭炎 张春雨[1] 刘宝权[1] 李建平[1] 王永志[1] Li Xiaoyu;Guo Yan;Zhang Chunyu;Liu Baoquan;Li Jianping;Wang Yongzhi(Jilin Academy of Agricultural Sciences,Gongzhuling 136100,China)

机构地区：[1]吉林省农业科学院,吉林公主岭136100

出　　处：《东北农业科学》2019年第6期53-56,共4页Journal of Northeast Agricultural Sciences

基　　金：农业部东北作物有害生物综合治理重点实验室开放基金课题(DB2018-09)。

摘　　要：本研究运用ELISA检测方法对吉林、黑龙江多个毛葱种植地区(柴岗、杨树林、哈拉海、前岗乡、三清山、太平山镇、肇州和阿城)的洋葱黄矮病毒(OYDV)感染情况进行了系统的调查。结果表明:八个地区中,OYDV感染情况都比较严重,整体检出率在30%以上,吉林太平山镇OYDV感染率最高,为75%,说明OYDV感染在东北毛葱种植区普遍存在;目测结果与ELISA检测结果符合率的分析发现,柴岗和肇州目测为健康的植株与OYDV-ELISA检测结果的符合率最高,为90%,哈拉海最低,只有10%,说明哈拉海地区种植的毛葱中有OYDV弱毒株感染而植株不显症现象;太平山镇和哈拉海目测为感病的植株与OYDVELISA检测结果阳性的符合率为100%。阳性植株鳞茎中OYDV分布情况为:外层和内层OYDV检出率低,中层检出率高。本研究明确了不同地区毛葱OYDV感染情况及OYDV在鳞茎中的分布,为毛葱的脱毒繁育奠定了理论基础。In this study, ELISA was used to investigate the infection of OYDV in part areas of Jilin and Heilongjiang Province. The results showed that in the eight regions(Chaigang, Yangshulin, Halahai, Qiangang, Sanqingshan, Taipingshan, Zhaozhou and Acheng), the OYDV infection situation was relatively serious, the OYDV infection incidence was more than 30%, and the infection rate of the OYDV at Taipingshan areas was 75%, which indicated that OYDV infection was prevalent in shallot growing areas in Northeast China. The coincidence rate between visual results and ELISA results was analyzed. The negative results of OYDV-ELISA and visual detection healthy results in Chaigang and Zhaozhou showed the highest coincidence rate(90%). Halahai was the lowest, for only 10%, which indicated OYDV attenuated strains infection in Harahai with no symptoms. The coincidence rate of symptomatic plant and the OYDV-ELISA-positive was 100% in Taipingshan and Halahai. The infection rate of OYDV in scales of positive bulb was low in outer layer and inner layer and high in middle layer. In this study, the infection of OYDV in different areas and its distribution in scale tablets were clarified, which laid the foundation for the virus-free and breeding of onion.

关 键 词：分蘖洋葱 洋葱黄矮病毒 

分 类 号：S633.9[农业科学—蔬菜学] S436.33[农业科学—园艺学]