蒙农红豆草EMS诱变后代光合特性比较研究  

Comparison on Photosynthetic Characteristics of EMS Mutagenesis Progeny of Mengnong Sainfoin

作　　者：乔雨 石凤翎 钱亚斯 张雨桐 闫伟 QIAO Yu;SHI Feng-ling;QIAN Ya-si;ZHANG Yu-tong;YAN Wei(Inner Mongolia Agricultural University,College of Grassland,Resources and Environment,Key Laboratory of Grassland Resources,Ministry of Education,Hohhot,Inner Mongolia 010019,China)

机构地区：[1]内蒙古农业大学,草原与资源环境学院,草地资源教育部重点实验室,内蒙古呼和浩特010019

出　　处：《草地学报》2020年第2期367-374,共8页Acta Agrestia Sinica

基　　金：内蒙古自治区应用技术研究与开发资金计划项目(201702072);内蒙古自治区草原英才“内蒙古高原乡土草种质资源遗传改良与利用”创新团队资助。

摘　　要：本研究以蒙农红豆草(Onobrychis viciifolia Scop‘Mengnong’)甲基磺酸乙酯(EMS,Ethyl Methane Sulfonate)诱变后代(M 1和M 2)和未经诱变(CK)为试验材料,采用逐步聚类法基于株高和SPAD值将M 1,M 2和CK群体分为A,B,C 3类,测定每个群体中不同类别材料光合日动态,筛选出具有高光合潜力的红豆草材料。结果表明:M 1,M 2和CK在15:00以后,净光合速率和气孔导度降低,胞间CO 2浓度上升,说明导致其光合作用降低的原因是非气孔因素。B类的M 2代净光合速率日均值最大;A,B,C 3类的M 2以及A类的M 1的气孔导度日均值最高;A类的CK和B类的CK和M 2的水分利用效率较大。总体而言,B类M 2代净光合速率(5.76μmol·CO 2·m-2·s-1)最高,水分利用效率(2.33μmol·mmol-1)相对较高。因此,EMS诱变蒙农红豆草M 2代的B类群体可作为高产育种的亲本材料。The experimental materials were EMS mutagenesis progeny M 1 and M 2 and CK without mutagenesis.The M 1,M 2 and CK populations were divided into A,B and C 3 groups.The diurnal dynamics of photosynthetic characteristics of different species in each group were measured to Screen sainfoin materials with high photosynthetic potential.The result showed:Pn and Gs were decreased but Ci increased in M 1,M 2 and CK populations after 15:00,which illustrated the decrease of photosynthesis of Mengnong Sainfoin was non-stomatal factor.Pn in type B in the M 2 population was the maximum in all categories,A and C in M 2 and B in CK were the next.Gs in types A,B and C in the M 2 population and classification of A in the M 1 were the maximum in all categories.The lowest Ci was in class A of the CK.The highest WUE was in class A of the CK.Therefore,the B group in the M 2 generation in Mengnong Sainfoin can be used as a parent material for high-yield breeding.

关 键 词：蒙农红豆草 突变群体 SPAD 净光合速率 

分 类 号：S816[农业科学—饲料科学]