不同饲养温度对草地贪夜蛾过冷却点和体液冰点的影响  

Effects of different feeding temperature on the supercooling points and freezing points of fall armyworm,Spodoptera frugiperda

作　　者：谢殿杰 张蕾[1] 程云霞[1] 江幸福[1] XIE Dianjie;ZHANG Lei;CHENG Yunxia;JIANG Xingfu(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

机构地区：[1]植物病虫害生物学国家重点实验室,中国农业科学院植物保护研究所,北京100193

出　　处：《植物保护》2020年第2期62-71,共10页Plant Protection

基　　金：中国农业科学院草地贪夜蛾联合攻关重大科技任务(Y2019YJ06);国家自然科学基金(31672019,31871951);国家绿肥产业技术体系(CARS-22);国家重点研发计划(2017YFD0201701,2017YFD0201802);北京市自然科学基金(6172030)。

摘　　要：为明确不同温度条件下草地贪夜蛾耐寒能力的差异,本研究利用过冷却点仪对不同温度条件下饲养的草地贪夜蛾各虫态的过冷却点和体液冰点进行了测定,并进行了频次分布分析。结果显示:在不同温度(16、20、24、28、32、36℃)条件下饲养的草地贪夜蛾,各虫态过冷却点和体液冰点存在显著差异。其中,3龄幼虫、6龄幼虫以及2日龄蛹的过冷却点和体液冰点均为在16℃下饲养时最低,3龄幼虫分别为(-13.48±0.53)℃和(-8.97±0.35)℃;6龄幼虫分别为(-8.94±0.28)℃和(-4.99±0.28)℃;2日龄蛹分别为(-18.09±0.50)℃和(-12.17±0.37)℃。5日龄蛹过冷却点在16℃饲养时最低,为(-17.76±0.49)℃,体液冰点在32℃和16℃饲养时最低,分别为(-11.72±0.56)℃和(-11.02±0.39)℃;成虫过冷却点在16℃饲养时最低,为(-17.87±0.55)℃,体液冰点在28℃和16℃饲养时最低,分别为(-11.23±0.61)℃和(-10.98±0.44)℃。本研究结果表明低温对草地贪夜蛾的耐寒能力具有一定的驯化作用,同时适当的高温也可以提高草地贪夜蛾的耐寒能力。该研究结果有助于预测草地贪夜蛾在我国的越冬分布区域,对其监测预警及防控具有重要意义。In order to investigate the cold tolerance of Spodoptera frugiperda under different feeding temperatures,the supercooling point and freezing point were measured by the supercooling point meter,and the frequency distribution was analyzed.The results showed that there were significant differences in the supercooling and freezing points of each age at different feeding temperatures(16,20,24,28,32,36℃).The supercooling points and freezing points of 3-instar,6-instar and 2-day-old pupae were the lowest at 16℃,which were(-13.48±0.53)℃and(-8.97±0.35)℃for 3-instar,(-8.94±0.28)℃and(-4.99±0.28)℃for 6-instar,(-18.09±0.50)℃and(-12.17±0.37)℃for 2-day-old pupae,respectively.The supercooling point of 5-day-old pupae was the lowest at 16℃,which was(-17.76±0.49)℃,and the freezing point was the lowest at 32℃and 16℃,which were(-11.72±0.56)℃and(-11.02±0.39)℃,respectively.The adult supercooling point was the lowest at 16℃,which was(-17.87±0.55)℃,and the freezing point were the lowest at 28℃and 16℃,which were(-11.23±0.61)℃and(-10.98±0.44)℃,respectively.The results of this research indicated that low temperature had a certain role in acclimating the cold tolerance of the S.frugiperda,and the appropriate high temperature could also improve the cold tolerance.The results are helpful to predict the overwintering distribution of S.frugiperda in China,and are of great significance for its monitoring,early warning,prevention and control.

关 键 词：草地贪夜蛾 饲养温度 过冷却点 体液冰点 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]