焦炉工外周血TH1/TH2型细胞因子表达与IFN-γ、IL-10 DNA甲基化分析  

TH1/TH2 type cytokine expression and DNA aberrant methylation analysis in peripheral blood of coke oven workers

作　　者：赵慧[1] 高艳荣[1] 宋统球 贾玉巧[1] Zhao Hui;Gao Yanrong;Song Tongqiu;Jia Yuqiao(School of Public Health,Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014060,China)

机构地区：[1]内蒙古科技大学包头医学院公共卫生学院,包头014060

出　　处：《卫生研究》2020年第2期242-248,共7页Journal of Hygiene Research

基　　金：包头医学院科学研究基金(No.BYJJ-YF 201617)。

摘　　要：目的了解焦炉逸散物(coke oven emissions,COEs)暴露的焦炉工多环芳烃(polycyclic aromatic hydrocarbons,PAHs)内暴露水平及焦炉工机体外周血白细胞介素-2(interleukin-2,IL-2)、γ-干扰素(interferon-γ,IFN-γ)、白细胞介素-4(IL-4)、白细胞介素-10(IL-10)的表达,及IFN-γ、IL-10表观遗传机制在COEs暴露损伤中的表现。方法随机选取某焦化厂炼焦车间接触COEs的85名工人作为暴露组,同是焦化厂非COEs暴露的47名作业工人作为对照组,采集暴露组及对照组工人晨尿进行碱水解-高效液相色谱荧光法检测1-羟基芘(1-OHPyr)水平,尿肌酐校正;采集空腹外周静脉血,用酶联免疫吸附试验测定血清中IL-2、IFN-γ、IL-4和IL-10的表达;采用飞行时间质谱分析IFN-γ和IL-10的甲基化水平。结果焦炉工人尿1-OHPyr含量高于对照组(F=12.446,P<0.05),且炉侧工和炉顶工高于对照组(P<0.05)。与对照组相比,焦炉工血清IL-2含量降低(F=14.774,P<0.05),焦炉工血清IFN-γ含量降低(F=46.379,P<0.05),焦炉工血清IL-4含量升高(F=17.426,P<0.05),焦炉工血清IL-10含量升高(F=33.515,P<0.05),焦炉工血清TH1/TH2比值降低(F=21.677,P<0.05)。在暴露组中,炉顶工IFN-γ水平高于炉底工,炉顶及炉底工IL-10水平低于炉侧工(P<0.05)。暴露组IL-10胞嘧啶(C)-磷酸(p)-鸟嘌呤(G)(CpG)-11、CpG-15和平均甲基化率低于对照组(P<0.05)。暴露组IFN-γCpG-5甲基化率高于对照组,差异有统计学意义。焦炉作业工人尿1-OHPyr含量与TH1/TH2比值、IFN-γ表达水平呈负相关,与IL-4、IL-10水平之间呈正相关。随着尿1-OHPyr浓度增加IL-10 CpG-11、CpG-15甲基化率有降低趋势。结论 COEs暴露的炉侧工与炉顶工为职业健康重点监护对象;焦炉工COEs暴露影响免疫调节细胞因子的表达;COEs暴露引起IL-10甲基化率改变。OBJECTIVE To understand the exposure levels of polycyclic aromatic hydrocarbons(PAHs) and the expression of interleukin-2(IL-2), interferon-γ(IFN-γ), interleukin-4(IL-4), interleukin-10(IL-10) in peripheral blood of coke oven workers exposed to coke oven emissions(COEs).The other purpose of this study was to understand the performance of IFN-γ and IL-10 epigenetic mechanisms in COEs exposure damage. METHODS The 85 workers exposed to COEs in a coking plant were randomly selected as the exposure group.The 47 workers who were exposed to non-COEs in the coking plant were used as the control group. The morning urine of the exposure group and the control group were subjected to detection of 1-OHPyr levels with alkaline hydrolysis High-performance liquid chromatography fluorescence,urine creatinine correction.The peripheral venous blood were subjected to detection of the expression of IL-2,IFN-γ,IL-4 and IL-10 with enzyme-linked immunosorbent assay.And methylation levels of IFN-γ and IL-10 were analyzed by time of flight mass spectrometry. RESULTS The urine 1-hyroxy-pyrene(1-OHPyr) content of coke oven workers was higher than that of the control group(F=12.446, P<0.05). The urine 1-OHPyr content of the furnace side and the furnace top were higher than the control group, and the differences were statistically significant. Compared with the control group, serum IL-2 content of coke oven workers decreased(F=14.774, P<0.05), and serum IFN-γ content of coke oven workers decreased(F=46.379, P<0.05), the serum IL-4 content of coke oven workers increased(F=17.426, P<0.05), the serum IL-10 content of coke oven workers increased(F=33.515, P<0.05), and the TH1/TH2 ratio of coke oven workers decreased(F=21.677, P<0.05). In the exposed group, the level of IFN-γ in the top of the furnace was higher than that in the bottom of the furnace. The difference was statistically significant. The level of IL-10 in the top and bottom of the furnace was lower than that in the furnace. The difference was statistically significant. The IL-1

关 键 词：焦炉逸散物 多环芳烃 1-羟基芘 细胞因子 DNA甲基化 

分 类 号：R135.1[医药卫生—劳动卫生]