天麻素对缺血缺氧脑损伤新生大鼠小胶质细胞Sirt3表达的影响  

The effects of gastrodin on the expression of Sirt3 in microglia of hypoxic-ischemic brain damage rats

作　　者：郭静 吴芳 袁云 李娟娟 Guo Jing;Wu Fang;Yuan Yun;Li Juanjuan(Department of Human Anatomy/Histology and Embryology,Faculty of Basic Medical Sciences,Kunming Medical University,Kunming 650500,China)

机构地区：[1]昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明650500

出　　处：《神经解剖学杂志》2020年第1期45-50,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31960194,31760297,31460274);2019年云南省科技厅-昆明医科大学应用基础研究联合专项重点项目,2019FE001(-003);云南省基础研究计划(昆医联合专项),2018FE001(-189)。

摘　　要：目的:研究天麻素对缺血缺氧脑损伤(HIBD)新生大鼠脑内激活的小胶质细胞Sirt3表达的影响。方法:选取39只3 d龄SD幼年大鼠随机分为对照组(control)、缺血缺氧脑损伤组(HIBD)、天麻素组(HIBD+gastrodin)。采用左侧颈总动脉结扎结合缺氧法建立新生大鼠缺血缺氧性脑损伤(HIBD)模型,使用免疫荧光染色观察HIBD模型后新生大鼠大脑胼胝体区小胶质细胞的分布情况;使用免疫荧光双标染色及Western Blot检测大鼠大脑左侧胼胝体区小胶质细胞Sirt3的表达变化。结果:免疫荧光染色结果显示HIBD后新生大鼠大脑左侧胼胝体区小胶质细胞出现明显激活,确定模型建立成功。免疫荧光双标染色及Western Blot结果均显示,与对照组相比,HIBD模型组Sirt3的表达水平明显升高(P<0.05);与HIBD模型组相比,天麻素组Sirt3的表达进一步增强(P<0.05)。结论:天麻素可能通过促进新生大鼠脑内小胶质细胞Sirt3的表达,发挥其神经保护作用。Objective:To study the effects of gastrodin on the expression of Sirt3 in microglia of hypoxic-ischemic brain damage neonatal rats.Methods:Thirty-nine 3-day-old SD rats were randomly divided into control group,model group(HIBD)and gastrodin group(HIBD+gastrodin).The hypoxic-ischemic brain damage(HIBD)model of neonatal rats was established by ligating the left carotid artery combined with hypoxia.Immunofluorescence staining was used to observe the distribution of microglia in the corpus callosum of neonatal rats after HIBD model.Immunofluorescence double-labeling staining and Western Blot were used to detect the expression of Sirt3 in the microglia of the left corpus callosum.Results:Immunofluorescence double staining showed that microglial cells in the left corpus callosum were activated after HIBD model,which proved the models were established successfully.Both immunofluorescence staining and Western Blot results showed that compared with the control group,the expression level of Sirt3 in the HIBD model group was significantly increased(P<0.05).Compared with the HIBD model group,the expression of Sirt3 in the gastrodin group was further enhanced(P<0.05).Conclusion:Gastrodin may exert its neuroprotective effects by promoting the expression of Sirt3 in microglia of neonatal rats.

关 键 词：小胶质细胞 缺血缺氧性脑损伤 天麻素 Sirt3 大鼠 

分 类 号：R285.5[医药卫生—中药学]