祛风消炎汤对类风湿关节炎大鼠滑膜组织Th17/Treg细胞转录因子RORγt和Foxp3表达影响的研究  

Effect of Qufeng Xiaoyan Decoction on Expression of Transcription Factors RORγt and Foxp3 in Synovial Tissue Th17/Treg cell of Rats with Rheumatoid Arthritis

作　　者：陈双双 孙建 朱科达 杭煜宇 鲁构峰 CHEN Shuangshuang;SUN Jian;ZHU Keda(Department of Rheumatic Immunology,Traditional Chinese Medicine Hospital of Zhangjiagang City,Zhangjiagang Jiangsu 215600,China)

机构地区：[1]张家港市中医医院风湿免疫科,江苏张家港215600

出　　处：《四川中医》2020年第3期85-88,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：江苏省卫生厅科研项目(编号:Z201204)。

摘　　要：目的:探究祛风消炎汤对类风湿关节炎(RA)大鼠滑膜组织Th17/Treg细胞转录因子RORγt和Foxp3表达影响的研究。方法:制备胶原诱导性关节炎(CIA)大鼠模型,分为模型组、祛风消炎汤低、中、高剂量组(10、20、40g/kg)及地塞米松组(2mg/kg),每组10只,另取10是正常大鼠作为对照组,共治疗35天。测定各组大鼠治疗前后足关节炎指数(AI)和右后足肿胀度,流式细胞术检测各组大鼠滑膜组织辅助性T细胞17(Th17)和调节性细胞T细胞(Terg)水平,RT-PCR法检测RORγt mRNA和Foxp3mRNA表达水平。结果:治疗后,相比较于模型组,祛风消炎汤能呈剂量依赖性降低CIA大鼠AI评分及足肿胀程度(P<0.05)。与模型组比较,祛风消炎汤低、中、高剂量组Treg细胞占比及Foxp3mRNA水平显著增加(P<0.05),Th17细胞占比、Th17/Treg比值及RORγt mRNA水平显著降低(P<0.05)。结论:祛风消炎汤对RA大鼠具有良好的治疗作用,其作用机制可能与通过降低RA大鼠滑膜组织RORγt水平,提升Foxp3水平来调节Th17/Treg平衡有关。Objective:To explore the effect of Qufeng Xiaoyan Decoction on the expression of transcription factors RORγt and Foxp3 in synovial tissue Th17/Treg cell of rats with rheumatoid arthritis(RA).Methods:Rat models of collagen-induced arthritis(CIA)were prepared and divided into model group,low,medium and high-dose Qufeng Xiaoyan Decoction groups(10,20,40 g/kg)and Dexamethasone group(2 mg/kg),with 10 rats/group.Another 10 normal rats were set as controlled group,and they were treated for 35 days.The arthritic index(AI)and right hind foot swelling degree were measured before and after treatment.The levels of synovial tissue helper T cell 17(Th17)and regulatory T cell(Terg)were detected by flow cytometry.The expression levels of RORγt mRNA and Foxp3 mRNA were detected by RT-PCR.Results:After treatment,compared with the model group,Qufeng Xiaoyan Decoction reduced the AI score and foot swelling degree in CIA rats in a dose-dependent manner(P<0.05).Compared with the model group,the proportion of Treg cell and the Foxp3 mRNA level were increased significantly in low,medium and high-dose Qufeng Xiaoyan Decoction groups(P<0.05),and the proportion of Th17 cell,Th17/Treg ratio and RORγt mRNA level were decreased significantly(P<0.05).Conclusions:Qufeng Xiaoyan Decoction has good therapeutic effects on RA rats,and its role mechanism may be related to the regulation of Th17/Treg balance by decreasing the RORγt level and increasing Foxp3 level in synovial tissue of RA rats.

关 键 词：祛风消炎汤 类风湿关节炎 滑膜组织 Th17 Treg 转录因子 

分 类 号：R285.5[医药卫生—中药学]