水凝胶三维培养对人羊膜间充质干细胞特性及旁分泌效应的影响  

Effects of hydrogel three-dimensional culture on the characteristics of human amniotic mesenchymal stem cells and paracrine effect

作　　者：王旗 杨晓双 王达利 Wang Qi;Yang Xiaoshuang;Wang Dali(Department of Plastic Surgery,Affiliated Hospital of Zunyi Medical University,Zunyi 563000,Guizhou Province,China)

机构地区：[1]遵义医科大学附属医院整形外科,贵州省遵义市563000

出　　处：《中国组织工程研究》2020年第22期3460-3466,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81560313),项目负责人:王达利;国家自然科学基金面上项目(81871570),项目负责人:王达利。

摘　　要：背景:研究表明间充质干细胞在创面愈合中具有减轻炎症反应、促进创面愈合、减轻瘢痕形成的作用,然而以往的普通二维培养环境由于存在细胞间接触性抑制,可能导致细胞的基因表达、信号传导和形态学存在差异。目的:观察人羊膜间充质干细胞旁分泌创面愈合相关因子的能力是否受二维培养环境与三维培养环境的影响。方法:利用传统酶消化法获得人羊膜间充质干细胞后,分别接种于普通细胞培养瓶(二维培养)与ShakeGelTM3D水凝胶中(三维培养),将水凝胶中的人羊膜间充质干细胞分别进行成脂、成骨、成软骨诱导分化,免疫荧光染色确定细胞分化方向;待两种培养环境中的人羊膜间充质干细胞融合至70%-80%,于倒置相差显微镜和激光共聚焦显微镜下观察细胞的生长特点及形态;培养24 h后,应用RT-qPCR技术测定细胞旁分泌创面愈合相关因子的mRNA相对表达量;培养48 h后,利用ELISA试剂盒检测细胞旁分泌创面愈合相关因子的蛋白表达量。结果与结论:①二维培养组人羊膜间充质干细胞呈扁平状,为典型间充质样细胞形态;三维培养组人羊膜间充质干细胞呈圆形,均匀分散于水凝胶的每一层;②三维培养中的人羊膜间充质干细胞具有3系诱导分化潜能;③三维培养组白细胞介素6、白细胞介素8、表皮生长因子、碱性成纤维细胞因子、透明质酸、肝细胞生长因子、血管内皮生长因子mRNA相对表达量高于二维培养组(P<0.001,P<0.05),两组白细胞介素4、白细胞介素10、金属蛋白酶组织抑制因子、基质金属蛋白酶、转化生长因子、角化细胞生长因子mRNA相对表达量比较差异无显著性意义(P>0.05);④三维培养组白细胞介素6、白细胞介素10、表皮生长因子、碱性成纤维细胞因子、白细胞介素8、肝细胞生长因子、转化生长因子β1、血管内皮生长因子蛋白表达量高于二维培养组(P<0.001,P<0.01,PBACKGROUND:Studies have shown that mesenchymal stem cells can reduce inflammation,promote wound healing,and reduce scar formation in wound healing.However,previous two-dimensional culture environment can lead to differences in gene expression,signal transduction,and morphology because of intracellular contact inhibition.OBJECTIVE:To investigate whether the ability of wound healing related factors secreted by human amniotic mesenchymal stem cells is affected by two-dimensional or three-dimensional culture environment.METHODS:The human amniotic mesenchymal stem cells cultured by traditional enzyme digestion method were inoculated in traditional cell culture flask(two-dimensional culture group)and ShakeGelTM 3D hydrogel(three-dimensional culture group)and induced to differentiate into adipocytes,osteoblasts,and chondrocytes,respectively.The direction of cell differentiation was determined by immunofluorescence staining.Human amniotic mesenchymal stem cells were fused to 70%-80%in two culture environments,and the growth characteristics and morphology of cells were observed under inverted phase contrast microscope and laser confocal microscope.After 24 hours of culture,relative mRNA expression of wound healing-related factors was detected by reverse transcription-quantitative polymerase chain reaction.After 48 hours of culture,the protein expression of wound healing-related factors was detected by the enzyme-linked immunosorbent assay.RESULTS AND CONCLUSION:(1)Human amniotic mesenchymal stem cells cultured in two-dimensional culture group were flat and spindle-shaped,which was a typical mesenchymal stem cell-like morphology.Human amniotic mesenchymal stem cells in the three-dimensional culture group were round and evenly dispersed in each layer of the hydrogel.(2)Human amniotic mesenchymal stem cells in the three-dimensional culture group exhibited the potential to differentiate into adipocytes,osteoblasts,and chondrocytes.(3)The mRNA expression of interleukin-6,interleukin-8,epidermal growth factor,basic fibroblast g

关 键 词：水凝胶 羊膜间充质干细胞 二维培养 三维培养 创面修复 旁分泌效应 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]