高强度间歇运动对自发性高血压模型大鼠病理性心脏肥大的影响及机制  

High-intensity interval training for treating pathological cardiac hypertrophy in spontaneously hypertensive rats:effects and mechanisms

作　　者：袁国强[1] 秦永生[2] 彭朋[2] Yuan Guoqiang;Qin Yongsheng;Peng Peng(Department of Physical Education,Zhengzhou University of Aeronautics,Zhengzhou 450015,Henan Province,China;Department of Health Service,Logistics University of Chinese People’s Armed Police Forces,Tianjin 300309,China)

机构地区：[1]郑州航空工业管理学院体育部,河南省郑州市450015 [2]中国人民武装警察部队后勤学院卫生勤务系,天津市300309

出　　处：《中国组织工程研究》2020年第23期3708-3715,共8页Chinese Journal of Tissue Engineering Research

基　　金：天津市自然科学基金项目(17JCYBJC274002),项目负责人:彭朋。

摘　　要：背景:规律运动具有健康促进效应,中低强度持续有氧运动已成为高血压等慢性病患者一级和二级预防的重要策略,然而高强度间歇运动的效果尚存在争议。目的:观察高强度间歇运动对自发性高血压大鼠病理性心脏肥大的作用并探讨其可能机制。方法:30只雄性自发性高血压大鼠随机分为对照组和运动组,同时将15只Wistar-Kyoto大鼠作为正常血压组。正常血压组和对照组大鼠保持安静状态,运动组进行8周高强度间歇运动。实验后利用无创血压仪测定血压,超声心动术检测心脏结构与功能,苏木精-伊红染色获取心肌细胞横截面积,RT-PCR法检测胚胎基因——心房钠尿肽和脑钠尿肽的mRNA表达量,Western blot法检测PI3-K、Akt、CnAβ和NFATc3的蛋白表达量。结果与结论:①与正常血压组比较,对照组血压升高(P<0.05),左心室出现向心性肥大(心腔缩窄、室壁增厚、心肌细胞横截面积升高),心功能下降(P<0.05),脑钠尿肽mRNA表达上调(P<0.05),CnAβ蛋白表达量升高(P<0.05)、p-NFATc3/t-NFATc3比值降低(P<0.05),PI3-K(p110α)蛋白和p-Akt/t-Akt比值无显著变化(P>0.05);②与对照组比较,运动组收缩压下降(P<0.05),心脏发生离心性肥大(心腔扩张),心功能升高(P<0.05),脑钠尿肽mRNA表达下调(P<0.05),PI3-K(p110α)蛋白和p-Akt/t-Akt比值升高(P<0.05),CnAβ蛋白以及p-NFATc3/t-NFATc3比值无显著变化(P>0.05);③结果表明,8周高强度间歇运动激活PI3-K/Akt信号转导途径诱导自发性高血压大鼠由病理性心脏肥大向生理性肥大转变并改善心功能,但并未对Cn/NFAT途径产生抑制效应。BACKGROUND:Regular exercise training possesses health promotion effect.Low-to-moderate intensity continuous aerobic exercise has been an important strategy for primary and secondary prevention of chronic diseases such as hypertension;however,the effect of high-intensity interval training is still debated.OBJECTIVE:To explore the effects of high-intensity interval training on pathological cardiac hypertrophy and investigate the possible mechanism in spontaneously hypertensive rats.METHODS:Thirty male spontaneously hypertensive rats were randomly assigned into a control group and a training group.Fifteen Wistar-Kyoto rats were used as normotensive group.Rats in the normotensive and control group were housed at rest,while those in the training group were subjected to a high-intensity interval training lasting for 8 weeks.After experiment,blood pressure was detected using a non-invasive blood pressure tester,and cardiac structure and function were measured by echocardiogram.Histopathological detection was performed by hematoxylin-eosin and Masson staining to determine myocardial cross-sectional area.mRNA expression of fetal genes including atrial natriuretic peptide and brain natriuretic peptide were detected by RT-PCR.Protein expression of PI3-K,Akt,CnAβand NFATc3 was detected using western blot assay.RESULTS AND CONCLUSION:Compared with the normotensive group,the blood pressure level was significantly elevated(P<0.05),concentric hypertrophy in the left ventricle appeared(including narrowing of the left ventricular cavity,ventricular wall thickening,increasing of myocardial cross-sectional area),cardiac function reduced significantly(P<0.05),mRNA of brain natriuretic peptide upregulated(P<0.05),CnAβprotein raised(P<0.05),p-NFATc3/t-NFATc3 ratio reduced(P<0.05),and PI3-K(p110α)protein and p-Akt/t-Akt ratio had no significant changes(P>0.05)in the control group.Compared with the control group,the blood systolic pressure was lowered(P<0.05),eccentric hypertrophy(cardiac chamber extension)in the left ventricle occurr

关 键 词：高强度间歇运动 高血压 心脏肥大 大鼠 信号转导途径 PI3-K/AKT Cn/NFAT 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]