窄谱中波紫外线联合吡美莫司乳膏对成人型特应性皮炎患者PBMC产生eotaxin和CCR3表达的影响  被引量:7

Effects of NB-UVB with pimecrolimus cream on the expression of eotaxin in serum and CCR3 on peripheral blood monocytes in patients with adult atopic dermatitis

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作  者:杨艳[1] 李嘉彦[1] 周欣[1] 田歆[1] 高爱莉[1] 熊斯颖[1] 李薇[1] 陈晓吟[1] 刘玉梅[1] 朱慧兰[1] YANG Yan;LI Jia-yan;ZHOU Xin;TIAN Xin;GAO Ai-li;XIONG Si-ying;LI Wei;CHEN Xiao-yin;LIU Yu-mei;ZHU Hui-lan(Guangzhou Institute of Dermatology,Guangzhou 510095,China)

机构地区:[1]广州市皮肤病防治所,广东广州510095

出  处:《皮肤性病诊疗学杂志》2020年第1期5-9,共5页Journal of Diagnosis and Therapy on Dermato-venereology

基  金:广东省医学科研基金资助课题(编号:A2018427);广州市医学卫生科技一般引导项目(编号:20171A011283)。

摘  要:目的:检测窄谱中波紫外线(NB-UVB)联合吡美莫司乳膏治疗前后,特应性皮炎(AD)患者外周血嗜酸性粒细胞活化趋化因子(eotaxin)与其相应受体CCR3的表达,以探讨其治疗特应性皮炎的相关机制。方法:采用窄谱中波紫外线联合吡美莫司乳膏治疗30例成人型AD患者,酶联免疫吸附试验检测治疗前后血清中eotaxin水平;同时用流式细胞仪分析外周血中CCR3的表达。结果:治疗前,AD患者血清eotaxin水平为(133.86±42.23)pg/mL,CCR3表达水平为(23.10±6.31)%;治疗后,AD患者血清eotaxin水平为(101.54±35.63)pg/mL,较治疗前明显降低(t=3.20,P<0.01);外周血CCR3表达水平为(16.52±6.59)%,较治疗前亦明显降低(t=3.59,P<0.01)。结论:窄谱中波紫外线联合吡美莫司乳膏可能通过降低eotaxin、CCR3表达,从而减少嗜酸性粒细胞的募集、活化,发挥其治疗特应性皮炎的作用。Objective:To compare expression levels of eotaxin in the serum and CCR3 on the peripheral blood monocytes(PBMC)prior to and after the treatment with NB-UVB plus pimecrolimus cream,in order to elucidate the pathogenesis of atopic dermatitis(AD).Methods:Serum level of eotaxin was detected by enzym-linked immunosorbent assay(ELISA)in 30 adult patients with AD prior to and after the treatment.CCR3 expression on the peripheral blood monocytes was analyzed by flow cytometry.Results:Treatment of AD with NB-UVB plus pimecrolimus cream markedly lowered the serum levels of eotaxin[(133.86±42.23)pg/mL vs.(101.54±35.63)pg/mL,P<0.01],and CCR3 on PBMC[(23.10±6.31)%vs.(16.52±6.59)%,P<0.01].Conclusions:Combination of NB-UVB with pimecrolimus cream could decrease the expression levels of eotaxin and CCR3,resulting in a reduction in recruitment and activation of eosinophils,consequently leading to improvement of AD.This study also suggested that combination of NB-UVB with pimecrolimus cream is safe and effective for AD.

关 键 词:皮炎 特应性 EOTAXIN CCR3 窄谱中波紫外线 吡美莫司乳膏 

分 类 号:R758.2[医药卫生—皮肤病学与性病学]

 

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