Q开关Nd:YAG 1064nm激光对表皮葡萄球菌活性及结构的影响  

Effects of Q-switch Nd:YAG 1064nm laser on the activity and structure of staphylococcus epidermidis

作　　者：谈桂其 钟静 翁智胜[1] 彭洁雯[1] 李晓辉[1] 廖梦怡[1] 易江华[1] 辛甜甜[1] TAN Gui-qi;ZHONG Jing;WENG Zhi-sheng;PENG Jie-wen;LI Xiao-hui;LIAO Meng-yi;YI Jiang-hua;XIN Tian-tian(Dermatology Department,The Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150,China)

机构地区：[1]广州医科大学附属第三医院皮肤科,广东广州510150

出　　处：《皮肤性病诊疗学杂志》2020年第1期10-13,共4页Journal of Diagnosis and Therapy on Dermato-venereology

摘　　要：目的:探讨不同能量密度的Q开关Nd:YAG 1064nm激光对表皮葡萄球菌的菌体活性及结构的影响。方法:临床获取毛囊炎标本,用胰蛋白胨大豆肉汤琼脂平板(TSA)培养获取菌落,基因测序进行鉴定,确定表皮葡萄球菌后进行培养,使用Q开关Nd:YAG 1064 nm激光对菌体进行照射。实验组:激光光斑直径2mm,脉宽≤8ns,根据不同能量密度15.9、19.1、22.2、25.4、28.7 J/cm^2分设5组;对照组不予激光照射。以菌落计数法评估菌体活性,并利用透射电镜观察菌体结构的改变。激光能量与菌落数的相关性采用Pearson相关分析。结果:实验组菌落计数分别为(234500±12554)、(89200±10485)、(52200±6214)、(26200±4157)、(8300±2213)CFU/mL,对照组菌落数为(276400±13150)CFU/mL,各组间差异有统计学意义(F=1536.97,P<0.05)。激光能量与菌落数呈负相关(r=-0.90,P<0.05)。能量密度在19.1 J/cm^2以上时,对菌体结构有破坏作用,能量越大,破坏程度越大。结论:Q开关Nd:YAG 1064nm激光对表皮葡萄球菌有抑菌和结构破坏作用。Objective:To investigate the effects of Q-switch Nd:YAG 1064nm laser at different energy levels on the activity and structure of staphylococcus epidermidis.Methods:Colonies of folliculitis were collected from patients,and cultured with TSA.Identification of staphylococcus epidermidis was made by gene sequencing.The staphylococcus epidermidis was cultured and irradiated by Q-switch Nd:YAG 1064nm laser.In the experimental group,staphylococcus epidermidis was irradiated with laser at a spot size of 2 mm diameter,pulse width≤8 ns,and various energy levels(15.9,19.1,22.2,25.4,28.7 J/cm^2),while staphylococcus epidermidis without laser treatment served as the control.The survivability and structure of staphylococcus epidermidis were evaluated by counting colony-forming unit and transmission electron microscopy,respectively.The pearson correlation analysis was used to determine the correlation of laser energy and number of colony.Results:Following irradiation with laser at energy levels of 15.9,19.1,22.2,25.4,28.7 J/cm^2,colony counts were 234500±12554,89200±10485,52200±6214,26200±4157,8300±213 CFU/mL,respectively,which were significantly lower than the control(276400±13150 CFU/mL)(F=1536.97,P<0.05).Colony counts negatively correlated with laser energy levels(r=-0.90,P<0.05).In addition,laser induced a dose-dependent disruption of bacterial structure,starting at energy levels above 19.1 J/cm 2.Conclusions:Q-switch Nd:YAG 1064nm laser can destroy the structure of staphylococcus epidermidis,and inhibit their activity.

关 键 词：Q开关ND:YAG 1064nm激光 表皮葡萄球菌 菌体活性 

分 类 号：R378.11[医药卫生—病原生物学]