改良小鼠胚胎下颌下腺的获取及其体外器官培养模型的建立  

Acquisition of submandibular gland in mouse embryo and establishment of an in vitro organ culture model

作　　者：刘志凯 乔翔鹤 苟黎明 李春洁[1] Liu Zhikai;Qiao Xianghe;Gou Liming;Li Chunjie(State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Dept.of Head and Neck Oncology,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China;Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences,Stomatological Hospital of Chongqing Medical University,Chongqing 401147,China)

机构地区：[1]口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医院头颈肿瘤外科,成都610041 [2]口腔疾病与生物医学重庆市重点实验室,重庆医科大学附属口腔医院,重庆401147

出　　处：《华西口腔医学杂志》2020年第2期228-232,共5页West China Journal of Stomatology

基　　金：国家自然科学基金(81870782);四川大学创新火花项目(2018SCUH0055)。

摘　　要：分支形态的形成对于保证器官可以在有限的体积内获得最有效的功能形态具有重要意义。下颌下腺是研究器官分支形态发生过程的重要模型,在小鼠胚胎中获取下颌下腺组织进行体外器官培养是一种重要的研究方法。本文介绍了一种改良的小鼠胚胎下颌下腺获取方法,并将整个获取及建立体外器官培养流程作以简介,以对其在体内发育过程中的分支形态发生过程进行更好体外的模拟,从而能够更好地开展相关研究。Branching morphology is important to ensure that the organ can obtain the efficient functional morphology in a limited volume.The submandibular gland is a crucial model for studying the morphological processes of organ branches.Harvesting the submandibular gland from mouse embryo is also an essential research technique.In this paper,a modified method for obtaining the submandibular glands of mouse embryo was introduced,and the whole process of obtaining and establishing in vitro organ culture was briefly introduced to accurately simulate branch morphogenesis for vivo development and related research.

关 键 词：小鼠 胚胎 下颌下腺 体外器官培养 分支形态发生 

分 类 号：Q813.1+3[生物学—生物工程]