Comparative analysis of diverse toxins from a new pharmaceutical centipede,Scolopendra mojiangica  

作　　者：Zi-Chao Liu Jin-Yang Liang Xin-Qiang Lan Tao Li Jia-Rui Zhang Fang Zhao Geng Li Pei-Yi Chen Yun Zhang Wen-Hui Lee Feng Zhao 

机构地区：[1]Key Laboratory of Ethnic Medical Resources Research and Southeast Asian International Cooperation of Yunnan Universities,Department of Biology and Chemistry,Puer University,Puer,Yunnan 665000,China [2]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan Province,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan 650223,China [3]Engineering Research Center for Exploitation and Utilization of Leech Resources in Universities of Yunnan Province,School of Agronomy and Life Sciences,Kunming University,Kunming,Yunnan 650214,China [4]Key Laboratory of Active Molecules and Drug Development,Puer University,Puer,Yunnan 665000,China [5]Kunming College of Life Science,University of Chinese Academy of Sciences,Kunming,Yunnan 650204,China [6]Institute of Comparative Study of Traditional Materia Medica,Institute of Integrative Medicine of Fudan University,Shanghai 200032,China [7]Nanshan College,Guangzhou Medical University,Guangzhou,Guangdong 511436,China

出　　处：《Zoological Research》2020年第2期138-147,共10页动物学研究（英文）

基　　金：supported by grants from the Chinese National Natural Science Foundation(81860696,31560596,81373945,and 31360516);Yunnan Applied Basic Research Projects(2016FD076);Key Research Program of the Chinese Academy of Sciences(KJZD-EW-L03);"Yunling Scholar"Program,Yunnan Provincial Training Programs of Youth Leader in Academic and Technical Reserve Talent(2019HB058);Puer University(2017XJKT12&CXTD011)。

摘　　要：As the oldest venomous animals,centipedes use their venom as a weapon to attack prey and for protection.Centipede venom,which contains many bioactive and pharmacologically active compounds,has been used for centuries in Chinese medicine,as shown by ancient records.Based on comparative analysis,we revealed the diversity of and differences in centipede toxin-like molecules between Scolopendra mojiangica,a substitute pharmaceutical material used in China,and S.subspinipes mutilans.More than 6000 peptides isolated from the venom were identified by electrospray ionization-tandem mass spectrometry(ESI-MS/MS)and inferred from the transcriptome.As a result,in the proteome of S.mojiangica,246 unique proteins were identified:one in five were toxin-like proteins or putative toxins with unknown function,accounting for a lower percentage of total proteins than that in S.mutilans.Transcriptome mining identified approximately 10 times more toxin-like proteins,which can characterize the precursor structures of mature toxinlike peptides.However,the constitution and quantity of the toxin transcripts in these two centipedes were similar.In toxicity assays,the crude venom showed strong insecticidal and hemolytic activity.These findings highlight the extensive diversity of toxin-like proteins in S.mojiangica and provide a new foundation for the medical-pharmaceutical use of centipede toxin-like proteins.As the oldest venomous animals, centipedes use their venom as a weapon to attack prey and for protection. Centipede venom, which contains many bioactive and pharmacologically active compounds,has been used for centuries in Chinese medicine, as shown by ancient records. Based on comparative analysis, we revealed the diversity of and differences incentipedetoxin-likemolecules between Scolopendra mojiangica, a substitute pharmaceutical material used in China, and S. subspinipes mutilans.More than 6 000 peptides isolated from the venom were identified by electrospray ionization-tandem mass spectrometry(ESI-MS/MS) and inferred from the transcriptome. As a result, in the proteome of S.mojiangica, 246 unique proteins were identified: one in five were toxin-like proteins or putative toxins with unknown function, accounting for a lower percentage of total proteins than that in S. mutilans.Transcriptome mining identified approximately 10 times more toxin-like proteins, which can characterize the precursor structures of mature toxinlike peptides. However, the constitution and quantity of the toxin transcripts in these two centipedes were similar. In toxicity assays, the crude venom showed strong insecticidal and hemolytic activity. These findings highlight the extensive diversity of toxin-like proteins in S. mojiangica and provide a new foundation for the medical-pharmaceutical use of centipede toxin-like proteins.

关 键 词：CENTIPEDE TOXINS PHARMACEUTICAL USE Proteotranscriptomic analysis 

分 类 号：R994.5[医药卫生—毒理学]