Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb  

作　　者：Ning Zheng Zhi-Zhong Wang Song-Wei Wang Fang-Jia Yang Xu-Tao Zhu Chen Lu Anne Manyande Xiao-Ping Rao Fu-Qiang Xu 

机构地区：[1]Center of Brain Science,State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,National Center for Magnetic Resonance in Wuhan,Key Laboratory of Magnetic Resonance in Biological Systems,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences,Wuhan,Hubei 430071,China [2]University of the Chinese Academy of Sciences,Beijing 100049,China [3]Department of Automation,School of Electrical Engineering,Zhengzhou University,Zhengzhou,Henan 450001,China [4]School of Life Science,Wuhan University,Wuhan,Hubei 430072,China [5]School of Human and Social Sciences,University of West London,Middlesex TW89GA,UK [6]Divisions of Biomedical Photonics,Wuhan National Laboratory for Optoelectronics,Wuhan,Hubei 430074,China

出　　处：《Zoological Research》2020年第2期148-156,共9页动物学研究（英文）

基　　金：supported by the National Natural Science Foundation of China(31400946,31771156,91632303/H09,91732304 and 31830035);Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32030200)。

摘　　要：The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system(MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by singlecolor labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis(BST), anterior cortical amygdalar area(ACo), medial amygdaloid nucleus(MeA), and posteromedial cortical amygdaloid area(PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system(MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by singlecolor labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis(BST), anterior cortical amygdalar area(ACo), medial amygdaloid nucleus(MeA), and posteromedial cortical amygdaloid area(PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.

关 键 词：Accessory OLFACTORY bulb Efferent projections RETROGRADE rAAVs Projection neuron labeling Dispersion characteristics CIRCUITRY basis 

分 类 号：Q42[生物学—神经生物学]