棉花花生间作田中花生蚜对吡虫啉代谢抗性机制初步研究  

Preliminary Study on Metabolic Resistance Mechanism of Aphis craccivora in Intercropping Field of Cotton and Peanut to Imidacloprid

作　　者：杨媛雪 李卓 段爱玲 薛超[1] 须志平[2] 赵鸣[1] Yang Yuanxue;Li Zhuo;Duan Ailing;Xue Chao;Xu Zhiping;Zhao Ming(Cotton Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,China;School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]山东棉花研究中心,济南250100 [2]华东理工大学药学院,上海200237

出　　处：《棉花学报》2020年第2期151-157,共7页Cotton Science

基　　金：山东省自然科学基金(ZR2019BC055);山东省农业科学院农业科技创新工程学科团队(CXGC2018E06)。

摘　　要：【目的】探讨棉花花生间作田中棉蚜(Aphis gossypii Glover)和花生蚜(Aphis craccivora Koch.)对吡虫啉抗性的差异以及花生蚜对吡虫啉代谢抗性的机制,以科学有效防治这一类害虫,避免其对吡虫啉的抗性快速发展。【方法】采集山东巨野和临清2个地区的棉花花生间作农田中的棉蚜和花生蚜,对这两个地区的地理种群和室内敏感品系进行了吡虫啉的室内毒力测定;使用增效剂顺丁烯二酸二乙酯(Diethyl maleate,DEM)、胡椒基丁醚(Piperomyl butoxide,PBO)和磷酸三苯酯(Triphenyl phosphate,TPP)3种增效剂进行了增效剂效果试验;并检测了2个地理种群和室内敏感品系花生蚜细胞色素P450单加氧酶(Cytochrome P450 monooxygenase,P450)、谷胱甘肽S-转移酶(Glutathione S-transferase,GST)和羧酸酯酶(Carboxylesterase,CarE)3种解毒代谢酶的活性。【结果】临清和巨野2个地区的棉蚜对吡虫啉抗性处于中等水平,抗性倍数分别为43.2倍和54.6倍;而花生蚜对吡虫啉则处于敏感或低水平抗性,2个种群抗性倍数分别为3.7倍和8.3倍。防治花生蚜的增效剂试验结果表明,巨野种群中,PBO、DEM对吡虫啉具有显著的增效作用,增效比分别达到3.63、1.95,而TPP不具有增效作用;在临清种群中,PBO对吡虫啉具有显著的增效作用,增效比达到3.05,而DEM和TPP不具有增效作用。对花生蚜的代谢酶活力测定发现,巨野种群与敏感品系相比,CarE活性没有显著差异,而P450和GST活性显著高于敏感品系;临清种群与敏感品系相比,CarE和GST活性无显著性差异,而P450活性显著高于敏感品系。【结论】间作农田中2种蚜虫对吡虫啉的敏感性差异较大,需要合理施药延缓抗药性的增强,同时推断P450和GST在花生蚜对吡虫啉的抗药性中发挥一定的作用。[Objective]This study aims to explore the difference of imidacloprid resistance between Aphid gossypii and Aphid craccivora in intercropping field of cotton and peanut,and the mechanism of imidacloprid resistance to A.craccivora,so as to scientifically control these pests and to effectively avoid the rapid development of the resistance to imidacloprid.[Method]Two A.gossypii field populations and two A.craccivora field populations were collected from intercropping field of cotton and peanut in Juye and Linqing county of Shandong province,China.The bioassay experiment with two A.gossypii field populations,the bioassay and synergism experiment in two A.craccivora field populations were performed by the leaf dipping method.In addition,the activities of carboxylesterase(CarE),glutathione S-transferase(GST)and cytochrome P450 monooxygenase(P450)were assayed in the susceptible strain and two field populations of A.craccivora.[Result]Two A.gossypii field populations from Linqing and Juye exhibited moderate levels of resistance to imidacloprid,with the resistance ratios of 43.2-and 54.6-fold,while two A.craccivora field populations from Linqing and Juye showed susceptible and low level of resistance to imidacloprid,with the resistance ratio of 3.7-and 8.3-fold,respectively.According to the synergistic experiments,PBO and DEM significantly synergized imidacloprid in A.gossypii field population of Juye with the synergistic ratio of 3.63-and 1.95-fold,respectively,and TPP had no effect on imidacloprid toxicity.In A.craccivora field population of Linqing,PBO significantly synergized imidacloprid with the synergistic ratio of 3.05-fold,and DEM and TPP had no effect on imidacloprid.Further enzyme activity tests revealed that the activities of P450 and GST in Juye A.craccivora population were significantly higher than susceptible strain,and the activity of CarE had no significant difference between Juye A.craccivora population and susceptible strain.However,the activity of P450 in Linqing A.craccivora population was higher than

关 键 词：棉蚜 花生蚜 间作农田 吡虫啉 代谢抗性 

分 类 号：S435.622.1[农业科学—农业昆虫与害虫防治] S435.652[农业科学—植物保护]