淫羊藿苷干预大鼠椎间盘源性腰痛的实验研究  

An experimental study of icariin for intervention of discogenic low back pain in rats

作　　者：李记天 马言 罗漫丽 LI Jitian;MA Yan;LUO Manli(Luoyang Orthopedic-Traumatological Hospital,Zhengzhou 450016,Henan,China)

机构地区：[1]河南省洛阳正骨医院/河南省骨科医院,河南郑州450016

出　　处：《中医正骨》2020年第3期7-13,共7页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：河南省中医药科学研究专项课题(2019ZYZD02)。

摘　　要：目的:探讨淫羊藿苷(icariin,ICA)干预大鼠椎间盘源性腰痛的效果及可能的作用机制。方法:将45只8周龄SPF级雄性SD大鼠随机分为5组,假手术组、生理盐水组、ICA 50 mg·kg^-1组、ICA 100 mg·kg^-1组各10只,塞来昔布组5只。生理盐水组、ICA 50 mg·kg^-1组、ICA 100 mg·kg^-1及塞来昔布组大鼠通过L_(4~5)和L_(5~6)椎间盘穿刺建立大鼠腰痛模型;假手术组仅显露椎间盘,不进行穿刺。自造模后第7天开始进行药物干预,至造模后第21天结束。ICA 50 mg·kg^-1组和ICA 100 mg·kg^-1组以ICA溶液进行灌胃(ICA溶于去离子水中),每天剂量分别为50 mg·kg^-1和100 mg·kg^-1;塞来昔布组以塞来昔布胶囊进行灌胃(塞来昔布胶囊粉剂溶于去离子水中),每天100 mg·kg^-1;生理盐水组以生理盐水灌胃,每天100 mg·kg^-1;假手术组常规饲养,不进行干预。从各组随机选取5只大鼠,分别于造模前、造模后第7天、第14天、第21天、第28天、第35天进行足底机械性疼痛阈值检测。造模后第35天,足底疼痛阈值测定结束后处死假手术组、生理盐水组、ICA 50 mg·kg^-1组和ICA 100 mg·kg^-1组的5只大鼠,以qRT-PCR法测定椎间盘组织中P物质(substance P,SP)mRNA和降钙素基因相关肽(calcitonin gene related peptide,CGRP)mRNA的含量。造模后第21天时,处死假手术组、生理盐水组、ICA 50 mg·kg^-1组和ICA 100 mg·kg^-1组其余的5只大鼠,以ELISA法测定大鼠椎间盘组织中细胞因子诱导的中性粒细胞趋化剂-1(cytokine-induced neutrophil chemoattractant-1,CINC-1)含量。结果:①足底机械性疼痛阈值。时间因素和分组因素存在交互效应(F=17.971,P=0.001)。5组大鼠的足底机械性疼痛阈值总体比较,差异有统计学意义,即存在分组效应(F=146.660,P=0.000)。造模前后不同时点间足底机械性疼痛阈值的差异有统计学意义,即存在时间效应(F=118.057,P=0.000)。假手术组足底机械性疼痛阈值随时间未见明显变化,一直处于�Objective:To explore the effects of icariin(ICA)on discogenic low back pain(DLBP)in rats and its possible mechanism of action.Methods:Forty-five 8-week-old SPF-grade male SD rats were selected and were randomly divided into sham-operated group(10),normal saline group(10),ICA 50 mg/kg group(10),ICA 100 mg/kg group(10)and celecoxib group(5).The lumbago models were built in rats of normal saline group,ICA 50 mg/kg group,ICA 100 mg/kg group and celecoxib group by puncturing L4/L5 and L5/L6 intervertebral disc,while the surgeries were performed on rats in sham-operated group to expose their intervertebral discs and no intervertebral disc puncture was performed.The drug intervention were performed on rats from day 7 to day 21 after modeling.The rats in ICA 50 mg/kg group were intragastric administrated with ICA solution(ICA was dissolved into deionized water)in daily dosages of 50 mg/kg.The rats in ICA 100 mg/kg group,celecoxib group and normal saline group were intragastric administrated with ICA solution,celecoxib solution(celecoxib capsule powders were dissolved into deionized water)and normal saline respectively in daily dosage of 100 mg/kg,while the rats in sham-operated group were bred normally and were not given any drug interventions.Five rats were randomly selected out from each group,and plantar mechanical pain threshold values were measured before modeling and at the 7 th,14 th,21 st,28 th and 35 th day after the modeling respectively.At the 35 th day after the modeling,the five rats in sham-operated group,normal saline group,ICA 50 mg/kg group and ICA 100 mg/kg group were executed after the last measurement of pelma pain threshold value and the contents of substance P(SP)mRNA and calcitonin gene related peptide(CGRP)mRNA in intervertebral disc tissues were measured by using qRT-PCR assays.At the 21 st day after the modeling,the other 5 rats in sham-operated group,normal saline group,ICA 50 mg/kg group and ICA 100 mg/kg group were executed,and the content of cytokine-induced neutrophil chemoattractant-1(CINC

关 键 词：腰痛 椎间盘退行性变 淫羊藿甙 大鼠 Sprague-Dawley 动物实验 

分 类 号：R285.5[医药卫生—中药学]